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After processing the rat plasma, the proteins in the plasma were separated by extracting the analytes with acetonitrile\u2013methanol (9:1, v\/v). The chromatographic column used was the UPLC HSS T3 column, and the mobile phase (methanol\u2013water with 0.1% formic acid) under a gradient elution profile was used to separate the four compounds, with elution times for each analyte being less than 5\u2009min. Electrospray ionization in positive\u2010ion mode and operating in multiple reaction monitoring mode was used for quantitative analysis. Crassicauline A, fuziline, karacoline, and songorine were administered to 48 rats (<jats:italic>n<\/jats:italic>\u2009=\u20096 per group) orally (5\u2009mg\/kg) and intravenously (0.5\u2009mg\/kg). The standard curves demonstrated excellent linearity in the range of 1\u20132500\u2009ng\/mL, wherein all r values were greater than 0.99. The UPLC\u2013MS\/MS method for the determination of crassicauline A, fuziline, karacoline, and songorine in rat plasma was successfully applied in determining their pharmacokinetics parameters, from which their oral bioavailabilities were calculated to be 18.7%, 4.3%, 6.0%, and 8.4%, respectively.<\/jats:p>","DOI":"10.1002\/bmc.5821","type":"journal-article","created":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T03:09:10Z","timestamp":1705115350000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Development of an ultra\u2010high\u2010performance liquid chromatography\u2013tandem mass spectrometry method for the simultaneous determination of crassicauline A, fuziline, karacoline, and songorine in rat plasma and application in their pharmacokinetics"],"prefix":"10.1002","volume":"38","author":[{"given":"Fan","family":"Chen","sequence":"first","affiliation":[{"name":"Ruian People's Hospital The Third 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