{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T18:43:43Z","timestamp":1767725023677,"version":"build-2238731810"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T00:00:00Z","timestamp":1705449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Determining a drug's bioavailability and bioequivalence is important for developing and approving a drug product. The procedure supports applications for generic drug products and novel therapeutic substances, makes important decisions regarding safety and efficacy, and measures a drug's concentration in biological matrices. This study aimed to develop and validate a specific, simple, sensitive, and accurate method using liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS) for measuring bumetanide (BUM) in human plasma. Chromatographic separation was achieved using a Hypurity C18 column (4.6\u00a0\u00d7\u200950\u2009mm, 5\u00a0\u03bcm) under isocratic conditions, and LC\u2013MS detected positive ionization acquisition modes. Protonated precursor to product ion transitions were observed at\n                    <jats:italic>m\/z<\/jats:italic>\n                    365.08\u2009\u2192\u2009240.10 and 370.04\u2009\u2192\u2009244.52 for BUM and internal standard, respectively. The linear range of BUM in plasma samples was 3.490\u2013401.192\u2009ng\/mL. The inter\u2010precision value ranged from 1.76% to 4.75%. The inter\u2010accuracy value ranged from 96.46% to 99.95%. The method was adequately validated per the U.S. Food and Drug Administration guidelines, and the results were within permissible bounds. The\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    and\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    values were ~53.097\u2009\u00b1\u200913.537\u2009ng\/mL and 1.25 (0.67\u20135.00)\u00a0h, respectively. The new approach showed satisfactory results for studying BUM in human plasma with potential use in pharmacokinetic and bioequivalence investigations.\n                  <\/jats:p>","DOI":"10.1002\/bmc.5825","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T01:20:16Z","timestamp":1705540816000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Liquid chromatography\u2013tandem mass spectrometry determination of bumetanide in human plasma and application to a clinical pharmacokinetic study"],"prefix":"10.1002","volume":"38","author":[{"given":"Ganesan Padmini","family":"Tamilarasi","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis, SRM College of Pharmacy SRMIST  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishnan","family":"Manikandan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, SRM College of Pharmacy SRMIST  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9863-4452","authenticated-orcid":false,"given":"Viswas Raja","family":"Solomon","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry MNR College of Pharmacy  Sangareddy India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.1.5794.211"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(88)80097-5"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.11.027"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/c4ay00255e"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000173128"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01868"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-2397-6_45"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00644735"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/39.10.425"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.02.018"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2021.108910"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2019.03.006"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2125.1996.00494.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.03.018"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123104"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)86078-8"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(91)80222-X"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.16.1124"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-30407-3"}],"updated-by":[{"DOI":"10.1002\/bmc.5958","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000}}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5825","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:50:49Z","timestamp":1760050249000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5825"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,17]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/bmc.5825"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5825","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,17]]},"assertion":[{"value":"2023-09-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5825"}}