{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:53:41Z","timestamp":1760061221702,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Super\u2010selective adrenal venous sampling (ssAVS) can collect the adrenal tributary venous blood in the aldosterone (ALD)\u2010hypersecreting segments in primary aldosteronism. The concentrations of the C18\u2010oxygenated steroids, especially 18\u2010oxocortisol (18\u2010oxoF), in the lesion segments might be more useful indices than those in the peripheral or adrenal central veins (current candidate indexes) for the differential diagnosis of unilateral ALD\u2010producing adenoma (APA) and bilateral adrenal hyperplasia (BAH). To verify this hypothesis, we developed a liquid chromatography\/electrospray ionization\u2010tandem mass spectrometry (LC\/ESI\u2013MS\/MS) method for simultaneously quantifying ALD, 18\u2010oxoF and 18\u2010hydroxycortisol in the adrenal tributary venous serum sample collected by ssAVS (ssAVS serum) and compared their concentrations between APA and BAH patients. Only deproteinization was required for a 10\u2009\u03bcl sample prior to the LC\/ESI\u2013MS\/MS analysis. Endogenous corticoids did not interfere with the quantifications, and the intra\u2010assay and interassay precisions (\u2264 8.3%) and accuracies (94.2\u2013102.7%) were acceptable. The clinical study revealed that the 18\u2010oxoF concentration was significantly higher in the ALD\u2010producing tumor tissues (from APA patients) than in the hyperplastic tissues (from BAH patients). However, in conclusion, the 18\u2010oxoF concentration in the ssAVS serum sample can be a rough indication but cannot be decisive for the differential diagnosis between APA and BAH owing to the significant individual difference.<\/jats:p>","DOI":"10.1002\/bmc.5841","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T17:53:46Z","timestamp":1707328426000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Determination of three C18\u2010oxygenated steroids in adrenal lesion segments in primary aldosteronism by super\u2010selective adrenal venous sampling and LC\/ESI\u2013MS\/MS"],"prefix":"10.1002","volume":"38","author":[{"given":"Yuka","family":"Mizumoto","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Tokyo University of Science  Noda Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayaka","family":"Hirakawa","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Tokyo University of Science  Noda Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Sugiura","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Keio University School of Medicine  Tokyo Japan"},{"name":"Multiomics Platform, Center for Cancer Immunotherapy and Immunobiology Kyoto University Graduate School of Medicine  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"Endocrinology &amp; 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