{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:49:11Z","timestamp":1791928151210,"version":"4.3.4"},"reference-count":18,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T00:00:00Z","timestamp":1712534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A sensitive and reliable LC\u2013MS\/MS method was developed and validated for the quantification of oxycodone and metabolites in human plasma. The method has a runtime of 6\u00a0min and a sensitivity of 0.1\u00a0\u03bcg\/L for all analytes. Sample preparation consisted of protein precipitation. Separation was performed on a Kinetix biphenyl column (2.1\u2009\u00d7\u2009100\u2009mm, 1.7\u00a0\u03bcm), using ammonium formate 5\u00a0m\n                    <jats:sc>m<\/jats:sc>\n                    in 0.1% aqueous formic acid and methanol LC\u2013MS grade 100% in gradient elution at a flow rate of 0.4\u00a0ml\/min. Detection was performed in multiple reaction monitoring mode using positive electrospray ionization. The method was linear over the calibration range of 0.1\u201325.0\u00a0\u03bcg\/L for oxycodone, noroxycodone and noroxymorphone and 0.1\u20135.0\u00a0\u03bcg\/L for oxymorphone. The method demonstrated good performance in terms of intra\u2010 and interday accuracy (86.5\u2013110.3%) and precision (CV 1.7\u20139.3%). The criteria for the matrix effect were met (CV\u2009&lt;\u200915%) except for noroxymorphone, for which an additional method was applied to compensate for the matrix effect. Whole blood samples were stable for 4\u00a0h at room temperature. Plasma samples were stable for 24\u2009h at room temperature and 3\u00a0months at \u221220\u00b0C. Furthermore, the method was successfully applied in a pharmacokinetic drug interaction study of oxycodone and enzalutamide in patients with prostate cancer.\n                  <\/jats:p>","DOI":"10.1002\/bmc.5874","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T06:09:40Z","timestamp":1712556580000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A rapid and sensitive LC\u2013MS\/MS method for quantifying oxycodone, noroxycodone, oxymorphone and noroxymorphone in human plasma to support pharmacokinetic drug interaction studies of oxycodone"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6948-4442","authenticated-orcid":false,"given":"Lotte M. G.","family":"Hulskotte","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacy Deventer Teaching Hospital  Deventer The Netherlands"},{"name":"Unit of PharmacoTherapy, \u2010Epidemiology &amp; \u2010Economics, Groningen Research Institute of Pharmacy University of Groningen  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge","family":"Wilbrink\u2010Pijffers","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy Deventer Teaching Hospital  Deventer The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurits E. L.","family":"Arbouw","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy Deventer Teaching Hospital  Deventer The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillemette E.","family":"Benoist","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy Deventer Teaching Hospital  Deventer The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank G. A.","family":"Jansman","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy Deventer Teaching Hospital  Deventer The Netherlands"},{"name":"Unit of PharmacoTherapy, \u2010Epidemiology &amp; \u2010Economics, Groningen Research Institute of Pharmacy University of Groningen  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge R. F.","family":"van Berlo\u2010van de Laar","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy Deventer Teaching Hospital  Deventer The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13112768"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-023-01255-1"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2165\/00128415-201214100-00010"},{"key":"e_1_2_10_5_1","unstructured":"European Medicines Agency. (2022).Committee for medicinal products for human use. ICH guideline M10 on bioanalytical method validation and study sample analysis. (Publication No. EMA\/CHMP\/ICH\/172948\/2019)."},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2015.11.035"},{"key":"e_1_2_10_7_1","unstructured":"IQVIA Institute. (2018).Global oncology trends 2018. Innovation expansion and disruption. (Publication No. PB.0017\u20101\u201005.2018)."},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-018-00731-3"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.2013.779669"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2008.64"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0b013e31816e2d4b"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e31819faa54"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/08998280.2000.11927719"},{"issue":"2","key":"e_1_2_10_14_1","first-page":"105","article-title":"Opioid complications and side effects","volume":"11","author":"Ramsin B.","year":"2008","journal-title":"Pain Physician"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.03.052"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2021.122625"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpainsymman.2015.12.340"},{"key":"e_1_2_10_19_1","unstructured":"World Health Organization. (2018).WHO guidelines for the pharmacological and radiotherapeutic management of cancer pain in adults and adolescents.Geneva. Licence: CC BY\u2010NC\u2010SA 3.0 IGO.\u00a0(Publication No. ISBN 978\u201092\u20104\u2010155039\u20100)."}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5874","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:37:09Z","timestamp":1760056629000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5874"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,8]]},"references-count":18,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/bmc.5874"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5874","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,8]]},"assertion":[{"value":"2023-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5874"}}