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This study aimed to explore the mechanism of CLMD in treating vertigo with anxiety disorder based on ultra\u2010performance liquid chromatography\u2013quadrupole time\u2010of\u2010flight\/mass spectrometry (UPLC\u2013Q\u2010TOF\/MS) and network pharmacology. UPLC\u2013Q\u2010TOF\/MS was performed to identify the compounds in blood and the targets of compounds of CLMD in vertigo and anxiety were searched using databases. A protein\u2013protein interaction network was built to screen the core targets. The core targets were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. In addition, the vertigo with anxiety rat model was used to verify the results. A total of 22 compounds were absorbed into the blood. Eighty\u2010one potential targets associated with CLMD for vertigo with anxiety disorder were identified through network pharmacological analysis. Subsequently, GO and KEGG analysis showed that CLMD treatment for vertigo with anxiety disorder is associated with neurotransmitter levels and other pertinent physiological processes. The results of the animal experiments showed that CLMD decreased the levels of serotonin, norepinephrine and dopamine, alleviating the symptoms of vertigo and anxiety disorder in model rats. The study revealed CLMD could alleviate vertigo and anxiety symptoms through reducing the levels of neurotransmitters.<\/jats:p>","DOI":"10.1002\/bmc.5881","type":"journal-article","created":{"date-parts":[[2024,5,19]],"date-time":"2024-05-19T21:38:31Z","timestamp":1716154711000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Combination of UPLC\u2013Q\u2010TOF\/MS and network pharmacology to reveal the mechanism of Chaihu\u2010jia\u2010Longgu\u2010Muli decoction for treating vertigo with anxiety disorder"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9692-3314","authenticated-orcid":false,"given":"Tong","family":"Wu","sequence":"first","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanshuo","family":"Dong","sequence":"additional","affiliation":[{"name":"Dizziness Clinic 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