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In this study, the constituents of XFBD granule, a granule dosage of XFBD prescription, were thoroughly examined <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> using liquid chromatography\u2013quadruple\u2010time\u2010of\u2010flight\u2013mass spectrometry, and the anti\u2010inflammatory constituents were screened. A total of 214 constituents were identified from the XFBD granule, 62 of which were confirmed via comparison with reference standards. After intragastric administration of XFBD granule, 63 and 28 constituents were absorbed into the rat sera and lungs in prototype form, respectively. XFBD granule and XFBD\u2010containing serum were found to significantly reduce nitric oxide (NO) and interleukin\u20106 (IL\u20106) secretion in lipopolysaccharide\u2010induced RAW264.7 cells. Five anti\u2010inflammatory constituents (verbasoside, scutellarin, luteolin, apigenin, and pogostone) were found to reduce the concentration of NO and IL\u20106 in a dose\u2010dependent manner. Moreover, the combination of these five constituents could significantly reduce NO secretion even when the concentration of each constituent was two to three orders of magnitude lower than their individual minimum effective concentrations. Overall, this study provides a valuable reference for the discovery of effective constituents from the XFBD granule.<\/jats:p>","DOI":"10.1002\/bmc.5898","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T23:23:32Z","timestamp":1716852212000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of Xuanfei Baidu granule constituents by liquid chromatography\u2013quadruple\u2010time\u2010of\u2010flight\u2013mass spectrometry and its anti\u2010inflammatory active constituents using a lipopolysaccharide\u2010induced RAW264.7 cell model"],"prefix":"10.1002","volume":"38","author":[{"given":"Haoran","family":"Li","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Sciences Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of 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