{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:40:49Z","timestamp":1790908849590,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,6,23]],"date-time":"2024-06-23T00:00:00Z","timestamp":1719100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81874364"],"award-info":[{"award-number":["81874364"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Xiakucao Oral Liquid (XKCOL) has been widely used for treating mammary gland hyperplasia and goiter in China. However, its pharmacokinetic data have been missing to date. To conduct its pharmacokinetic study, we established an LC\u2013tandem mass spectrometry method for the simultaneous determination of eight XKCOL\u2010related compounds in rat plasma. Liquid\u2013liquid extraction was used for the sampling process. Chromatographic separation was performed on a Phenomenon Luna C<jats:sub>18<\/jats:sub> column with a mobile phase of methanol and 2\u2009mM ammonium acetate, using gradient elution at a flow rate of 0.8\u2009mL\/min. Detection was performed in the multiple reaction monitoring mode using negative electrospray ionization (ESI<jats:sup>\u2212<\/jats:sup>) with optimized MS parameters. Endogenous substances and carryover did not interfere in the detection of analytes. The calibration curves showed a good linear relationship within the linear ranges. The intra\u2010 and inter\u2010batch accuracy and precision were 94.8%\u2013110.0% and \u226411.2%, respectively. There was no significant matrix effect and the recovery was reproducible. The dilution of samples did not affect the accuracy and precision. The solution and plasma samples were stable under the various test conditions. The major components of XKCOL absorbed into the blood were salvianic acid A and rosmarinic acid. They demonstrated linear kinetics over the dose range used in this study.<\/jats:p>","DOI":"10.1002\/bmc.5902","type":"journal-article","created":{"date-parts":[[2024,6,23]],"date-time":"2024-06-23T22:28:51Z","timestamp":1719181731000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development and validation of a liquid chromatography\u2013tandem mass spectrometry method for simultaneous quantification of eight Xiakucao Oral liquid\u2013related compounds in rat plasma and its application in pharmacokinetic study"],"prefix":"10.1002","volume":"38","author":[{"given":"Jingyi","family":"Jin","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Pharmacokinetics Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Xu","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pharmacokinetics Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medicine Guiyang XinTian Pharmaceutical Co., LTD  Guiyang China"},{"name":"Department of Medicine Haitian Medical Technology Co., LTD  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medicine Guiyang XinTian Pharmaceutical Co., LTD  Guiyang China"},{"name":"Department of Medicine Haitian Medical Technology Co., LTD  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Medicine Guiyang XinTian Pharmaceutical Co., LTD  Guiyang China"},{"name":"Department of Medicine Haitian Medical Technology Co., LTD  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongguo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medicine Haitian Medical Technology Co., LTD  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3451-5446","authenticated-orcid":false,"given":"Dongming","family":"Yan","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pharmacokinetics Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Furong","family":"Qiu","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pharmacokinetics Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0251649"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2003.11.028"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.02.047"},{"key":"e_1_2_7_5_1","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Chinese Pharmacopoeia Commission","year":"2020"},{"issue":"8","key":"e_1_2_7_6_1","first-page":"2082","article-title":"Expert consensus statement on treatment of goiter\/nodular thyroid disease with Xiakucao Oral liquid in clinical practice","volume":"51","author":"Experts of Clinical Application of Xiakucao Oral Liquid","year":"2020","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.19540\/j.cnki.cjcmm.20190314.001"},{"issue":"7","key":"e_1_2_7_8_1","first-page":"394","article-title":"Antibacterial activity and mechanism of extracts from Prunellae Spica against pathogenic organisms of acne","volume":"12","author":"Hu D. 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