{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T04:59:43Z","timestamp":1777179583209,"version":"3.51.4"},"reference-count":10,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T00:00:00Z","timestamp":1716422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>VX\u2010548 is an orally active and highly selective NaV 1.8 inhibitor that is undergoing development for the treatment of acute pain. The aim of this study was to develop a liquid chromatography\u2010tandem mass spectrometric (LC\u2013MS\/MS) method for the measurement of VX\u2010548 in monkey plasma. VX\u2010548 was extracted from the plasma using acetonitrile\u2010mediated protein precipitation. The quantitative analysis was performed on a Thermo Vantage TSQ mass spectrometer with ibrutinib as an internal standard. Chromatography was performed on a Waters ACQUITY UPLC BEH C<jats:sub>18<\/jats:sub> column with 0.1% aqueous formic acid and acetonitrile as mobile phase. The precursor\u2010to\u2010product ion transitions were <jats:italic>m\/z<\/jats:italic> 474.2\u2009&gt;\u2009165.0 and <jats:italic>m\/z<\/jats:italic> 441.2\u2009&gt;\u2009138.1 for VX\u2010548 and internal standard, respectively. This developed method was successfully validated in the concentration range of 1\u20131000\u2009ng\/mL. The calibration curve showed excellent linearity with a correlation coefficient of &gt;0.999. The precision expressed as relative standard deviation (RSD) was &lt;8.4%, whereas the accuracy denoted as relative error (RE) ranged from \u22125.0% to 9.1%. The mean recovery was &gt;84%. VX\u2010548 was stable in monkey plasma after storage under certain conditions. The validated method was successfully applied to the pharmacokinetic study of VX\u2010548 in monkey plasma after single oral (2\u2009mg\/kg) and intravenous (1\u2009mg\/kg) administrations.<\/jats:p>","DOI":"10.1002\/bmc.5907","type":"journal-article","created":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T00:23:28Z","timestamp":1716510208000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A simple and sensitive ultra\u2010high performance liquid chromatography tandem mass spectrometry method for the quantitative analysis of VX\u2010548 in monkey plasma: Method validation and application to pharmacokinetic study"],"prefix":"10.1002","volume":"38","author":[{"given":"Huiying","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmacy Xuzhou Central Hospital  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonggang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Xuzhou Central Hospital  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Xuzhou Central Hospital  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1514-2998","authenticated-orcid":false,"given":"Wenfei","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Xuzhou Central Hospital  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/379257a0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1999.0828"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00052.2017"},{"key":"e_1_2_6_5_1","volume-title":"Guidance for industry: Bioanalytical method validation","author":"Food and Drug Administration","year":"2018"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2209870"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2020.113210"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2018.12.016"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2001.86.2.629"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2014.10.037"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2015.01.025"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5907","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:37:38Z","timestamp":1760056658000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,23]]},"references-count":10,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/bmc.5907"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5907","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,23]]},"assertion":[{"value":"2024-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5907"}}