{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T03:36:58Z","timestamp":1784691418058,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008901","name":"College of Pharmacy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple, accurate and precise method was developed for the simultaneous estimation of the bempedoic acid and ezetimibe in pure and tablet dosage form. The developed method was validated as per International Conference on Harmonization guidelines. The chromatographic separation was achieved isocratically on a Waters\u2010 C<jats:sub>18<\/jats:sub>, 250\u2009\u00d7\u20094.6\u00a0mm, 5\u00a0\u03bcm column. Mobile phase containing K<jats:sub>2<\/jats:sub>HPO<jats:sub>4<\/jats:sub>\u2013methanol in the ratio 60:40 in buffer at pH 4.3 was pumped through column at a flow rate of 1.0\u00a0ml\/min. The temperature was maintained at 25\u00b0C. The optimized wavelength selected was 242\u2009nm. The separation of bempedoic acid and ezetimibe showed retention times of 3.090 and 4.268\u2009min respectively. The RSD values of the bempedoic acid and ezetimibe were 0.34 and 0.08 respectively. The accuracy of method was determined at three levels (50,100 and 150%). The percentage recovery was obtained as 100.0 and 100.0% for bempedoic acid and ezetimibe, respectively. The limits of determination and quantitation obtained from regression equations of bempedoic acid and ezetimibe were 1.065, 3.550 and 0.203, 0.677, respectively. The regression equation of bempedoic acid is <jats:italic>y<\/jats:italic>\u2009=\u200920,795<jats:italic>x<\/jats:italic>\u2009+\u200924,168, and it is <jats:italic>y<\/jats:italic>\u2009=\u20096,885.7<jats:italic>x<\/jats:italic>\u2009+\u200911,000 for ezetimibe. The retention times were decreased and the run time was decreased, so that the method developed is simple and economical that can be adopted for regular quality control tests in industry.<\/jats:p>","DOI":"10.1002\/bmc.5938","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T04:59:10Z","timestamp":1719377950000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Analytical method development and validation for simultaneous estimation of Bempedoic acid and Ezetimibe in pure and its pharmaceutical dosage form by RP\u2010HPLC"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6076-7062","authenticated-orcid":false,"given":"Aakash","family":"Suresh","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis JKKMMRFs\u2013Annai JKK Sampoorani Ammal College of Pharmacy, The Tamil Nadu Dr. MGR Medical University  Namakkal Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anbarasi","family":"Balakrishnan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis JKKMMRFs\u2013Annai JKK Sampoorani Ammal College of Pharmacy, The Tamil Nadu Dr. MGR Medical University  Namakkal Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijayamirtharaj","family":"Ramaswamy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis JKKMMRFs\u2013Annai JKK Sampoorani Ammal College of Pharmacy, The Tamil Nadu Dr. MGR Medical University  Namakkal Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senthilkumar","family":"Natesan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis JKKMMRFs\u2013Annai JKK Sampoorani Ammal College of Pharmacy, The Tamil Nadu Dr. MGR Medical University  Namakkal Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,26]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Bempedoic acid: A novel agent"},{"key":"e_1_2_7_3_1","volume-title":"Bempedoic acid|C19H36O5|CID 10472693"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11883-016-0611-4"},{"key":"e_1_2_7_5_1","volume-title":"The British Pharmacopoeial commission","author":"British Pharmacopoeia","year":"2011"},{"key":"e_1_2_7_6_1","first-page":"1","volume-title":"Validation of analytical procedures; methodology","author":"Code (Q2B)","year":"1996"},{"key":"e_1_2_7_7_1","volume-title":"A text book of pharmaceutical analysis","author":"Connors K. 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