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In this study, the liquid chromatography\u2013mass spectrometry (LC\u2013MS) method has been established to study the components of Epimedium and to identify the components absorbed into the blood of rats. Bioinformatics was used to screen out potential toxic components, and the integrating metabolomics method was used to explore the molecular mechanism of Epimedium\u2010induced liver injury. The chemical constituents of Epimedium were identified by LC\u2013MS, and 62 compounds were obtained, including 57 flavonoids, four organic acids and one alkaloid. The toxicity network of \u201cEpimedium\u2013component\u2013target\u2013liver injury\u201d was constructed using bioinformatics research methods, and then the key hepatotoxic component icaritin was identified. Integrating metabolomics was used to investigate the changes in the metabolic profile of L\u201002 cells with different durations of icaritin administration compared with the control group, and 106 different metabolites were obtained. A total of 14 potential biomarkers significantly associated with cell survival were screened by Pearson correlation analysis combined with the L\u201002 cell survival rate. Our study preliminarily revealed the mechanism of hepatotoxicity induced by Epimedium.<\/jats:p>","DOI":"10.1002\/bmc.5948","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T23:10:01Z","timestamp":1720566601000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Integrating metabolomics and bioinformatics to reveal the mechanism of Epimedium\u2010induced liver injury"],"prefix":"10.1002","volume":"38","author":[{"given":"Jiaqi","family":"Wang","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijia","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"},{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Georgia Institute of Technology  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0262-7830","authenticated-orcid":false,"given":"Tonghua","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengyuan","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoran","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3649-2428","authenticated-orcid":false,"given":"Chenning","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"},{"name":"Department of Pharmacy, Xiangyang No. 1 People's Hospital Hubei University of Medicine  Xiangyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5943-5119","authenticated-orcid":false,"given":"Yikun","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,9]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"80","article-title":"Guard against the risk of liver injury caused by Xianlinggubao oral preparation. 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