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It exhibits diverse pharmacological properties, including antioxidant, anti\u2010hyperuricemic, and anti\u2010inflammatory effects. The objective of this study was to develop a novel HPLC\u2010fluorescence (HPLC\u2010FL) method for the quantitative analysis of scopoletin in the plasma and to investigate its pharmacokinetics in rats. Sample preparation involved a methanol\u2010based protein precipitation method, and chromatographic separation was conducted using a C<jats:sub>18<\/jats:sub> column with an isocratic mobile phase composed of water and acetonitrile containing 0.1% trifluoroacetic acid. The eluent was detected using an FL detector set to optimized excitation\/emission wavelengths of 337\/453\u2009nm. Method validation encompassed assessments of selectivity, linearity (1\u2013500\u2009ng\/mL), precision, accuracy, recovery, matrix effect, and stability in accordance with the prevailing Food and Drug Administration (FDA) guidelines. The developed method was successfully applied for pharmacokinetic\u00a0study in rats. To the best of our knowledge, this study is the first application of a simple and sensitive HPLC\u2010FL method for the quantification of scopoletin in a pharmacokinetic study. This method offers a promising alternative for preclinical pharmacokinetic investigations with appropriate modifications and validations and holds potential for clinical applications.<\/jats:p>","DOI":"10.1002\/bmc.5959","type":"journal-article","created":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T01:39:21Z","timestamp":1721698761000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A simple and sensitive high\u2010performance liquid chromatographic method combined with fluorescence detection for bioanalysis of scopoletin in rat plasma: Application to a pharmacokinetic study"],"prefix":"10.1002","volume":"38","author":[{"given":"Taeyoung","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Manufacturing Pharmacy, College of Pharmacy and Research Institute for Drug Development Pusan National University  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong\u2010Gyun","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Manufacturing Pharmacy, College of Pharmacy and Research Institute for Drug Development Pusan National University  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0997-5723","authenticated-orcid":false,"given":"In\u2010Soo","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Manufacturing Pharmacy, College of Pharmacy and Research Institute for Drug Development Pusan National University  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.12.031"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4975"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123399"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40005-020-00505-9"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2005-837789"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9ra06056a"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/07352689.2011.616039"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.114589"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113141"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2019.05.023"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/19390210802519671"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-0835-2301"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40005-021-00551-x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2003.12.021"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/np300824h"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4678"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.07.130"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1639619"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/734824"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.04.044"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2022.106202"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2017.1335726"},{"issue":"7","key":"e_1_2_7_24_1","first-page":"S204","article-title":"Morinda citrifolia Linn. for prevention of postoperative nausea and vomiting","volume":"93","author":"Prapaitrakool S.","year":"2010","journal-title":"Journal of the Medical Association of Thailand"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcme.2015.09.003"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.22047"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2020.122508"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph14070610"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2020.113143"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2006.04.005"},{"key":"e_1_2_7_31_1","unstructured":"US Food and Drug Administration (US FDA). 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