{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T05:09:18Z","timestamp":1788239358059,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A liquid chromatography electrospray ionization tandem mass spectrometry method with amoxicillin\u2010d\n                    <jats:sub>4<\/jats:sub>\n                    as the stable isotope\u2010labeled internal standard for simultaneous quick detection of amoxicillin and clavulanic acid in human plasma was developed and validated. Chromatographic separations were performed on a Hedera ODS\u20102 column (2.1\u2009\u00d7\u2009150\u2009mm, 5\u00a0\u03bcm). The mobile phases for gradient elution were aqueous solution containing 0.2% acetic acid (AA) (mobile phase A) together with organic phase solution (acetonitrile and methanol mixed solution, mobile phase B). Mass spectrometry was performed using negative electrospray ionization in multiple reaction monitoring mode. The target fragment ion pairs of amoxicillin, clavulanic acid and amoxicillin\u2010d\n                    <jats:sub>4<\/jats:sub>\n                    were\n                    <jats:italic>m\/z<\/jats:italic>\n                    364.1\u2009\u2192\u2009223.1, 198.1\u2009\u2192\u2009135.9 and 368.1\u2009\u2192\u2009227.1, respectively. The linear ranges of this method were 40\u20135,000\u2009ng\/ml for amoxicillin and 30\u20132,500\u2009ng\/ml for clavulanic acid, with coefficient of determination &gt;\u20090.9900. This method validation included selectivity, standard curve, lower limit of quantitation, accuracy, precision, recovery, matrix effect (hemolytic matrix and hyperlipidemic matrix), carryover, stability, dilution reliability and incurred sample reanalysis study. A successful application of this method was realized in a pharmacokinetic study after administration of amoxicillin\u2013clavulanic acid potassium granules.\n                  <\/jats:p>","DOI":"10.1002\/bmc.5964","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T03:18:56Z","timestamp":1725938336000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["SIL\u2010IS LC\u2013ESI\u2013MS\/MS method for simultaneous quick detection of amoxicillin and clavulanic acid in human plasma: Development, validation and its application to a pharmacokinetics study"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2431-9350","authenticated-orcid":false,"given":"Jianbang","family":"Wu","sequence":"first","affiliation":[{"name":"Anhui Provincial Center of Drug Clinical Evaluation Yijishan Hospital of WannanMedical College  Wuhu Anhui People's Republic of China"},{"name":"School of pharmacy Wannan Medical College  Wuhu Anhui People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changmao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of pharmacy Wannan Medical College  Wuhu Anhui People's Republic of China"},{"name":"The People's Hospital of Lezhi  Ziyang Sichuan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hainan Simcere Pharmaceutical Co., Ltd.  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