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We obtained YG\u2010containing serum from Sprague\u2013Dawley rats, compared it with control group serum and analyzed it using gas chromatography\u2013mass spectroscopy to identify potential biomarkers and investigate the mechanism of YG in improving spermatogenic function in rats. Six important differential biomarkers, comprising putrescine, amidine, arginine,\n                    <jats:sc>d<\/jats:sc>\n                    \u2010fructose\u20106\u2010phosphate,\n                    <jats:sc>l<\/jats:sc>\n                    \u2010proline and galactose, were identified in the YG\u2010containing serum and then used to explore the potential mechanisms. The ultra\u2010high\u2010performance liquid chromatography\u2013high\u2010resolution mass spectrometry technology was adopted for the rapid separation, identification and analysis of chemical components of YG in blood. A total of 69 detected chromatographic peaks were revealed. The binding energy between core compounds and key proteins is low, among which dipsacoside B is the best. The outcomes suggest that YG may improve spermatogenic function in rats by facilitating the development of spermatogonial stem cells, counteracting oxidative stress and controlling cellular apoptosis. Youjing granule may also affect the energy required for sperm production or influence sperm growth and maturation.\n                  <\/jats:p>","DOI":"10.1002\/bmc.5967","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T06:52:13Z","timestamp":1724741533000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolomics combined with network pharmacology to investigate the pharmacodynamic components and potential mechanisms of the spermatogenic function of the Youjing granule"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8401-5952","authenticated-orcid":false,"given":"Mingxin","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Medicine The Affiliated Yixing Clinical School of Medical School of Yangzhou University  Yixing China"},{"name":"Department of Pharmacy The Affiliated Yixing Hospital of 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