{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T15:14:41Z","timestamp":1788794081757,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Withania somnifera<\/jats:italic>\n                    <\/jats:styled-content>\n                    belongs to the family Solanaceae, commonly called ashwagandha, and is traditionally used as an astringent, hepatoprotective and antioxidant, and as a treatment for rheumatism. Therefore the current study aimed to explore the dichloromethane fraction of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>W. somnifera<\/jats:italic>\n                    <\/jats:styled-content>\n                    whole plant (DCFWS) and ethyl acetate fraction of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>W. somnifera<\/jats:italic>\n                    <\/jats:styled-content>\n                    (EAFWS) using gas chromatoghraphy\u2013mass spectrometry (GC\u2013MS) analysis and to find the acetylcholinesterase inhibition potential along with spasmolytic activity. The GC\u2013MS\u2010detected phytochemicals were 2,4\u2010\n                    <jats:italic>bis<\/jats:italic>\n                    (1,1\u2010dimethylethyl), hexadecanoic acid, 1\u2010nonadecene and 11\u2010octadecenoic acid. The DCFWS and EAFWS exhibited acetylcholinesterase inhibitory potential with significant inhibitory concentration values. The acute toxicity results of both fractions showed high toxicity, causing emesis at 0.5\u00a0g and both emesis and diarrhea at 1\u00a0g\/kg. Both fractions exhibited significant (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.01) laxative activity against metronidazole (7\u00a0mg\/kg) and loperamide hydrochloride (4\u00a0mg\/kg) induced constipation. Both DCFWS (66.8\u2009\u00b1\u20093.85%) and EAFWS (58.58\u2009\u00b1\u20093.28%) significantly (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.05) increased charcoal movement compared with distal water (43.93\u2009\u00b1\u20094.34%). Similarly the effect of DCFWS on KCl\u2010induced (80\u2009m\n                    <jats:sc>m<\/jats:sc>\n                    ) contraction was more significant as compared with EAFWS. It was concluded that the plant can be used in the treatment of gastrointestinal tract diseases such as constipation. Furthermore, additional work is required in the future to determine the bioactive compounds that act as therapeutic agents in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>W. somnifera<\/jats:italic>\n                    <\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/bmc.5968","type":"journal-article","created":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T00:28:40Z","timestamp":1721694520000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phytochemical profile and pharmacological potential of\n                    <scp>\n                      <i>Withania somnifera<\/i>\n                    <\/scp>\n                    whole plant extracts"],"prefix":"10.1002","volume":"38","author":[{"given":"Ayaz Ali","family":"Sher","sequence":"first","affiliation":[{"name":"Department of Botany Islamia College Peshawar  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arshad","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Department of Botany Islamia College Peshawar  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Adil","sequence":"additional","affiliation":[{"name":"Center for Plant sciences and Biodiversity University of Swat  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zamarud","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Abdul Wali Khan University  Mardan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahid Ali","family":"Butt","sequence":"additional","affiliation":[{"name":"Department of Botany GC Women University Sialkot  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Botany University of Peshawar  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9458-3873","authenticated-orcid":false,"given":"Muhammad","family":"Nafees","sequence":"additional","affiliation":[{"name":"Department of Botany University of Peshawar  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saima","family":"Sohni","sequence":"additional","affiliation":[{"name":"Institute of Chemical Sciences University of Peshawar  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,22]]},"reference":[{"issue":"1","key":"e_1_2_11_2_1","first-page":"20","article-title":"Medicinal uses of chemical extracts from Withania somnifera and its antimicrobial activity: A Mini\u2010review","volume":"2","author":"Ali K.","year":"2017","journal-title":"PSM Microbiology"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-11-99"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-13-96"},{"issue":"1","key":"e_1_2_11_5_1","first-page":"30","article-title":"Therapeutic properties of medicinal plants: A review of plants with anticancer activity (part 1)","volume":"6","author":"Al\u2010Snafi A. 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