{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T20:03:08Z","timestamp":1767902588521,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T00:00:00Z","timestamp":1722988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Euphorbiae pekinensis<\/jats:italic> Radix (EPR) is a traditional Chinese herb commonly used to treat edema, pleural effusion, and ascites. However, counterfeit and adulterated products often appear in the market because of the homonym phenomenon, similar appearance, and artificial forgery of Chinese herbs. This study comprehensively evaluated the quality of EPR using multiple methods. The DNA barcode technique was used to identify EPR, while the UPLC\u2010Q\u2010TOF\u2010MS technique was utilized to analyze the chemical composition of EPR. A total of 15 tannin and phenolic acid components were identified. Furthermore, UPLC fingerprints of EPR and its common counterfeit products were established, and unsupervised and supervised pattern recognition models were developed using these fingerprints. The backpropagation artificial neural network and counter\u2010propagation artificial neural network models accurately identified counterfeit and adulterated products, with a counterfeit ratio of more than 25%. Finally, the contents of the chemical markers 3,3\u2032\u2010di\u2010O\u2010methyl ellagic acid\u20104\u2032\u2010O\u2010\u03b2\u2010D\u2010glucopyranoside, ellagic acid, 3,3\u2032\u2010di\u2010O\u2010methyl ellagic acid\u20104\u2032\u2010O\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010xylopyranoside, and 3,3\u2032\u2010di\u2010O\u2010methyl ellagic acid were determined to range from 0.05% to 0.11%, 1.95% to 8.52%, 0.27% to 0.86%, and 0.10% to 0.42%, respectively. This proposed strategy offers a general procedure for identifying Chinese herbs and distinguishing between counterfeit and adulterated products.<\/jats:p>","DOI":"10.1002\/bmc.5978","type":"journal-article","created":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T04:50:11Z","timestamp":1723006211000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification of <i>Euphorbiae pekinensis<\/i> Radix and its counterfeit and adulterated products based on DNA barcode, UPLC\u2010Q\u2010TOF\u2010MS, UPLC fingerprint, and chemometrics"],"prefix":"10.1002","volume":"38","author":[{"given":"Guangjiao","family":"You","sequence":"first","affiliation":[{"name":"College of Pharmacy Hebei University of Chinese Medicine  Shijiazhuang 050200 China"},{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin 301617 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangjie","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hebei University of Chinese Medicine  Shijiazhuang 050200 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyun","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hebei University of Chinese Medicine  Shijiazhuang 050200 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaonan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hebei University of Chinese Medicine  Shijiazhuang 050200 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lizhi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin 301617 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4566-9317","authenticated-orcid":false,"given":"Lili","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin 301617 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4246-7883","authenticated-orcid":false,"given":"Xiaoliang","family":"Ren","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin 301617 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemolab.2010.10.010"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2012.12.027"},{"key":"e_1_2_7_4_1","first-page":"519","article-title":"Effects of diterpenoid pekinenal of Euphorbia pekinensis Rupr. on proliferation, cell cycle and apoptosis of hepatoma cells","volume":"32","author":"Chen F.","year":"2016","journal-title":"Chinese Pharmacological Bulletin"},{"key":"e_1_2_7_5_1","article-title":"In vitro nephrotoxicity induced by herb\u2010herb interaction between Radix Glycyrrhizae and Radix Euphorbiae Pekinensis","volume":"2000","author":"Chen M.","year":"2000","journal-title":"Oxidative Medicine and Cellular Longevity"},{"key":"e_1_2_7_6_1","first-page":"236","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Chinese Pharmacopoeia Committee","year":"2020"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00424"},{"key":"e_1_2_7_8_1","first-page":"816","article-title":"Herbal textual research on Daji and Jingdaji","volume":"32","author":"He L.","year":"2009","journal-title":"Journal of Chinese Medicinal Materials"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201300389"},{"key":"e_1_2_7_10_1","first-page":"143","article-title":"Analysis of chemical components in supernatant and precipitate of Sanajon oral liquid based on UPLC\u2010Q\u2010TOF\u2010MSE technology","volume":"26","author":"Huang H.","year":"2020","journal-title":"Chinese Journal of Experimental Traditional Medical Formulae"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.01.041"},{"key":"e_1_2_7_12_1","first-page":"1348","article-title":"Rapid analysis and identification of chemical components of ethanol extract from Mongolian medicine Sanzi san by HPLC\u2010Q\u2010Exactive\u2010MS","volume":"33","author":"Li J.","year":"2022","journal-title":"China Pharmacy"},{"key":"e_1_2_7_13_1","first-page":"129","article-title":"Progress in chemical and pharmacological research of Euphorbia pekinensis Rupr","volume":"21","author":"Liu S.","year":"2019","journal-title":"Modern Chinese Medicine"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116619"},{"key":"e_1_2_7_15_1","first-page":"52","article-title":"Discussion on varieties of Euphorbiae pekinensis Radix","volume":"6","author":"Meng Q.","year":"2007","journal-title":"Tianjin Pharmacy"},{"key":"e_1_2_7_16_1","first-page":"657","article-title":"Identification and characterization of chemical constituents in cortex Juglandis Mandshuricae based on UPLC\u2010Q\u2010TOF\/MS","volume":"48","author":"Sun G.","year":"2017","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.08.003"},{"key":"e_1_2_7_18_1","first-page":"157","article-title":"Analysis of chemical constituents in Jiechangyan Qixiao granules based on UPLC\u2010Q\u2010TOF\/MSE","volume":"27","author":"Sun Y.","year":"2021","journal-title":"Chinese Journal of Experimental Traditional Medical Formulae"},{"key":"e_1_2_7_19_1","first-page":"1630","article-title":"Ellagic acid provokes DNA damage in human HEK\u2010293 cells","volume":"29","author":"Wang D.","year":"2013","journal-title":"Chinese Journal of Public Health"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22030465"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.01.016"},{"key":"e_1_2_7_22_1","first-page":"1277","article-title":"Chemical constituent cluster of decoction of Sanguisorbae radix by HPLC\u2010IT\u2010TOF\/MS","volume":"49","author":"Xu W.","year":"2018","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(02)00452-2"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2017.11.006"},{"key":"e_1_2_7_25_1","first-page":"251","article-title":"Characterization of chemical constituents from fruits of Rosa xanthina by UPLC\u2010Q\u2010TOF\u2010MS","volume":"42","author":"Zan L.","year":"2021","journal-title":"Science and Technology of Food Industry"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2017.12.019"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2019.125275"},{"key":"e_1_2_7_28_1","first-page":"254","article-title":"Analysis on chemical constituents from Terminalia chebula Retz. And Terminalia bellerica (Gaerth.) Roxb. By UPLC\u2010Q\u2010Exactive quadrupole\u2010Orbitrap\u2010mass spectrometry","volume":"41","author":"Zhou K.","year":"2020","journal-title":"Journal of Chinese Mass Spectrometry Society"},{"key":"e_1_2_7_29_1","first-page":"4422","article-title":"Statistical analysis on phenomenon of homonym and synonym of Chinese materia medica","volume":"30","author":"Zhu Y.","year":"2015","journal-title":"China Journal of Traditional Chinese Medicine and Pharmacy"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5978","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:19:55Z","timestamp":1760037595000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5978"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,7]]},"references-count":28,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1002\/bmc.5978"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5978","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,7]]},"assertion":[{"value":"2024-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5978"}}