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Tenacissoside G (Tsd\u2010G), a major active component of <jats:italic>Marsdenia tenacissima<\/jats:italic>, exhibits anticancer activity. To analyze the effect of Tsd\u2010G on the pharmacokinetics of paclitaxel (PTX), researchers selected 30 Sprague\u2013Dawley (SD) rats, randomized into a solvent control group, a verapamil positive control group, and 20, 40, and 60\u2009mg\/kg Tsd\u2010G groups. After seven consecutive days of intraperitoneal injection of verapamil or Tsd\u2010G, a single dose of 6\u2009mg\/kg PTX was injected intravenously. Plasma samples were collected at different time points, and proteins were precipitated using a methanol\u2013acetonitrile solution. An ultrahigh\u2010performance liquid chromatography\u2013tandem mass spectrometry method was developed, with docetaxel as an internal standard, and quantified using positive ion multiple reaction monitoring (MRM) mode. This analytical method's specificity, accuracy, precision, recovery, matrix effect, and sample stability meet the requirements for biological sample determination. After Tsd\u2010G administration in rats, the mean residence time of PTX was significantly prolonged. And Tsd\u2010G can stably bind to P\u2010gp by forming hydrogen bonds and inhibiting the expression of P\u2010gp in rat liver. Although the metabolites of PTX were not detected in this study, the above results still indicate the existence of HDIs between Tsd\u2010G and PTX, and P\u2010gp may be the main target to mediate HDIs.<\/jats:p>","DOI":"10.1002\/bmc.5984","type":"journal-article","created":{"date-parts":[[2024,8,17]],"date-time":"2024-08-17T06:16:35Z","timestamp":1723875395000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["P\u2010glycoprotein\u2010mediated herb\u2013drug interaction evaluation between Tenacissoside G and paclitaxel"],"prefix":"10.1002","volume":"38","author":[{"given":"Jiudong","family":"Hu","sequence":"first","affiliation":[{"name":"College of Food Science and Technology Shanghai Ocean University  Shanghai China"},{"name":"Department of Pharmacy Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 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