{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:34:50Z","timestamp":1786977290722,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100002429","name":"Amgen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002429","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Small molecule inhibitors (SMIs) are increasingly being used in the treatment of non\u2010small cell lung cancer. To support pharmacokinetic research and clinical treatment monitoring, our aim was to develop and validate an ultra\u2010performance liquid chromatography\u2013mass spectrometry (UPLC\u2010MS\/MS) assay for quantification of eight SMIs: adagrasib, alectinib, brigatinib, capmatinib, crizotinib, lorlatinib, selpercatinib, and sotorasib. Development of the UPLC\u2010MS\/MS assay was done by trying different columns and eluents to optimize peak shape. The assay was validated based on guidelines of the European Medicines Agency. Chromatographic separation was performed with a gradient elution using ammonium formate in water and methanol. Detection was performed using a triple quadrupole tandem mass spectrometer with electrospray ionization. Validation was performed in a range of 10\u20132500\u2009\u03bcg\/L for lorlatinib, 25\u20136250\u2009\u03bcg\/L for alectinib and crizotinib, 25\u201310,000\u2009\u03bcg\/L for capmatinib and selpercatinib, 50\u201312,500\u2009\u03bcg\/L for brigatinib, and 100\u201325,000\u2009\u03bcg\/L for adagrasib and sotorasib. Imprecision was &lt;8.88% and inaccuracy was &lt;12.5% for all compounds. Seven out of eight compounds were stable for 96\u2009h at room temperature. Sotorasib was stable for 8\u2009h at room temperature. A sensitive and reliable method has been developed to quantify eight SMIs with a single assay, enhancing efficacy and safety of targeted therapies.<\/jats:p>","DOI":"10.1002\/bmc.5986","type":"journal-article","created":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T05:19:53Z","timestamp":1723526393000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development and validation of an ultra\u2010performance liquid chromatography\u2013tandem mass spectrometry method to quantify the small molecule inhibitors adagrasib, alectinib, brigatinib, capmatinib, crizotinib, lorlatinib, selpercatinib, and sotorasib in human plasma"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5616-2064","authenticated-orcid":false,"given":"Esther M.","family":"Hollander","sequence":"first","affiliation":[{"name":"Department of Pharmacy and Research Institute for Medical Innovation Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nachel M. C.","family":"Zimmerman","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Research Institute for Medical Innovation Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berber","family":"Piet","sequence":"additional","affiliation":[{"name":"Department of Pulmonology Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel M.","family":"van den Heuvel","sequence":"additional","affiliation":[{"name":"Department of Pulmonology Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Burger","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Research Institute for Medical Innovation Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsey H. M.","family":"te Brake","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Research Institute for Medical Innovation Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2185-8201","authenticated-orcid":false,"given":"Rob","family":"ter Heine","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Research Institute for Medical Innovation Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmsacl.2023.05.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/7574369"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/mgg3.1398"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2018.02.016"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/d1an01928g"},{"key":"e_1_2_7_7_1","unstructured":"European Medicines Agency (EMA). (2023).Guideline on bioanalytical method validation.https:\/\/www.ema.europa.eu\/en\/documents\/scientific-guideline\/ich-guideline-m10-bioanalytical-method-validation-step-5_en.pdf"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4768"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12051196"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD013257.pub2"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5628"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2016-0068"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.5582\/ddt.2023.01043"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(16)31473-8"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2016.10.010"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2019.06.034"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2024.02.008"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2009.24.1.1"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.15321"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2023.123918"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/9028309"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.11.060"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.05.052"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2021.122718"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5720"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.04.035"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.08.038"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2012.08.021"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2018.03.014"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-023-01293-9"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5224"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2019.03.011"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpt.787"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5052"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2017.10.007"},{"key":"e_1_2_7_36_1","unstructured":"WHO. (2022).Cancer. Retrieved 5 December fromhttps:\/\/www.who.int\/news-room\/fact-sheets\/detail\/cancer"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2022-0173"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2021.122752"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5986","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:19:39Z","timestamp":1760037579000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5986"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,13]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1002\/bmc.5986"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5986","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,13]]},"assertion":[{"value":"2024-05-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5986"}}