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In this study, network pharmacology combined with RNA\u2010seq strategy was employed to predict the targets and pathways of GLXB for MI. GLXB significantly modulated signaling pathways related to the pathology of MI, such as anti\u2010inflammatory and anti\u2010apoptotic signaling pathways such as WNT, PI3K\/AKT, and AMPK. GSEA showed that GLXB administration downregulated these key pathways. In addition, Metabolomic analysis demonstrated that GLXB treatment reversed metabolic disorder. Integrative analysis demonstrated three key metabolites (pyruvate, lactate, and palmitate) and three differential genes (Pck1, Cdo1, and Cth) that affected glycolysis or gluconeogenesis and cysteine and methionine metabolism. The results of molecular docking showed that chrysin\u20107\u2010<jats:italic>O<\/jats:italic>\u2010glucuronide and diosmetin\u20107\u2010<jats:italic>O<\/jats:italic>\u2010rutinoside may be the crucial components that exert myocardial protective activity. Western blot showed that GLXB administration reversed the expression levels of Pck1, Cdo1, Cth, Alb, Bcl2, and Ccnd1. This study has elucidated that GLXB could alleviate MI in rats by modulating WNT and PI3K\/AKT signaling pathways, thereby reducing inflammation and apoptosis as well as improving energy metabolism.<\/jats:p>","DOI":"10.1002\/bmc.6007","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T20:34:05Z","timestamp":1727382845000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Revealing the mechanism of Gualou\u2013Xiebai against myocardial ischemia based on network pharmacology and energy metabolism strategies"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9038-0281","authenticated-orcid":false,"given":"Qi","family":"Jiang","sequence":"first","affiliation":[{"name":"College of Pharmacy Hubei University of Chinese Medicine  Wuhan 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