{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T07:37:49Z","timestamp":1779003469125,"version":"3.51.4"},"reference-count":13,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Huperzine A is a reversible and selective cholinesterase inhibitor and has been approved for the treatment of Alzheimer's diseases. In this study, we developed a highly sensitive and specific ulta\u2010high\u2010performance liquid chromatography\u2013tandem mass spectrometry method for the determination of Huperzine A in rat plasma. An aliquot of 50\u2009\u03bcL of rat plasma sample was pretreated with 200\u2009\u03bcL of acetonitrile\u2010methanol (\n                    <jats:italic>v\/v<\/jats:italic>\n                    ; 1:1) containing 0.2% formic acid followed by solid phase extraction. The resulting sample was separated on a Waters ACQUITY BEH C\n                    <jats:sub>18<\/jats:sub>\n                    column using acetonitrile and water containing 0.2% formic acid as mobile phase, at a flow rate of 0.3\u00a0mL\/min. Multiple\u2010reaction monitoring (MRM) mode was used for quantitative analysis of Huperzine A in positive electrospray ionization. In the concentration range of 0.01\u201310\u00a0ng\/mL, Huperzine A showed excellent linearity with correlation coefficient &gt; 0.998. The intra\u2010 and inter\u2010day RSD% were less than 9.7%, while the RE% ranged from \u22126.7% to 10.0%. The mean recovery was &gt;84.5%. The validated method was demonstrated to be selective, sensitive, and reliable, which has been successfully applied to pharmacokinetic study of Huperzine A in rat plasma. Huperzine A displayed a long half\u2010life in rat plasma and high oral bioavailability.\n                  <\/jats:p>","DOI":"10.1002\/bmc.6011","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T23:19:25Z","timestamp":1726787965000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development and validation of a highly sensitive UPLC\u2013MS\/MS method for the determination of Huperzine A in rat plasma"],"prefix":"10.1002","volume":"38","author":[{"given":"Kejun","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Neurology Xuzhou New Health Geriatric Hospital  Xuzhou Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9584-9416","authenticated-orcid":false,"given":"Haizhou","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology Xuzhou New Health Geriatric Hospital  Xuzhou Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.681532"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ijnp\/pyv098"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-014-9384-y"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26216531"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2006.12.010"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26030728"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1384"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03191002"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2102949118"},{"key":"e_1_2_6_11_1","unstructured":"U.S. Food and Drug Administration. (2018).Guidance for bioanalytical method validation.https:\/\/www.fda.gov\/media\/70858\/download"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.01.013"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.10.027"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074916"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.6011","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.6011","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.6011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T08:58:09Z","timestamp":1772960289000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.6011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,19]]},"references-count":13,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/bmc.6011"],"URL":"https:\/\/doi.org\/10.1002\/bmc.6011","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,19]]},"assertion":[{"value":"2024-08-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e6011"}}