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However, the bioactive components and comprehensive mechanisms underlying the therapeutic effects remain unclear. The present study investigates the potential active ingredients and underlying mechanisms of HGW against spermatogenesis dysfunction. The chemical components of HGW were analyzed by mass spectrometry. And then the \u201ccomponents\u2010targets\u2010pathway\u2010disease\u201d network was constructed using network pharmacology research methods, which aimed to identify the key active components and potential targets of HGW in treating oligospermia. Experimental validation was finally conducted in animal model. The male\u2010specific pathogen\u2010free Kunming mice were divided into five groups: Sham group, Model group, and HGW groups (8, 16, and 32\u2009g\/kg of HGW by gavage for 35\u2009days). Chemical profile and network pharmacology results revealed that potential bioactive compounds were dihydrocinnacasside, isomucronulatol, and 6\u2010gingerol, and the mechanism of which was enriched in regulating spermatogenic stem cells (SSCs), endocrine function, and apoptosis. The administration of HGW significantly improved oligospermia in mice. HGW significantly upregulated the expression of marker proteins in SSCs and the potential targets within the testis simultaneously. Our data indicates that HGW enhances the proliferation of SSCs, and HGW can be a promising therapeutic candidate for oligospermia.<\/jats:p>","DOI":"10.1002\/bmc.6023","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T03:19:48Z","timestamp":1728616788000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Huangqi\u2010Guizhi\u2010Wuwutang protects against oligospermia in mice by promoting the proliferation of spermatogenic stem cells: A comprehensive study using HPLC\u2010Q\u2010TOF\/MS and experimental pharmacology"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7646-6961","authenticated-orcid":false,"given":"Yuan","family":"Zhao","sequence":"first","affiliation":[{"name":"The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine  Shenzhen 518033 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