{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:47:14Z","timestamp":1760060834082,"version":"build-2065373602"},"reference-count":5,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T00:00:00Z","timestamp":1731456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple, accurate, and robust reverse\u2010phase high\u2010performance liquid chromatography (RP\u2010HPLC) method was developed and validated for the quantification of Baclofen in rat plasma. The method demonstrated high degree of linearity (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.9936) across a concentration range of 10\u201350\u2009\u03bcg\/mL. Precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), and robustness were evaluated according to ICH guidelines. The LOD and LOQ were found to be 0.076197 and 0.23090\u2009\u03bcg\/mL, respectively. This method provides an efficient approach for Baclofen quantification in plasma, making it suitable for pharmacokinetic and bioavailability studies. The novelty of this study lies in its optimization for routine use in laboratories, ensuring reproducibility with minimal variations across different conditions and analysts.<\/jats:p>","DOI":"10.1002\/bmc.6038","type":"journal-article","created":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T19:53:43Z","timestamp":1731527623000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel analytical approach for baclofen quantification in rodent plasma"],"prefix":"10.1002","volume":"39","author":[{"given":"Bindu","family":"Dhiman","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Lovely Professional University  Phagwara Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4243-714X","authenticated-orcid":false,"given":"Mithlesh","family":"Yadav","sequence":"additional","affiliation":[{"name":"G.D. Memorial College of Pharmacy  Jodhpur Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anju","family":"Dhiman","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Maharshi Dayanand University  Rohtak Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4702-6534","authenticated-orcid":false,"given":"Saurabh","family":"Satija","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Lovely Professional University  Phagwara Punjab India"},{"name":"School of Medical, Indigenous and Health Sciences University of Wollongong  Wollongong NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00013-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(03)00352-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.11.031"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.08.030"},{"volume-title":"Q2(R1) validation of analytical procedures: Text and methodology: Guidance for industry","year":"2005","author":"U.S. Food and Drug Administration","key":"e_1_2_8_6_1"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.6038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:58:10Z","timestamp":1760036290000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.6038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,13]]},"references-count":5,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/bmc.6038"],"URL":"https:\/\/doi.org\/10.1002\/bmc.6038","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2024,11,13]]},"assertion":[{"value":"2024-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e6038"}}