{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T19:07:51Z","timestamp":1788030471918,"version":"build-2784847793"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003785","name":"Guangdong Medical Research Foundation","doi-asserted-by":"publisher","award":["B2020033"],"award-info":[{"award-number":["B2020033"]}],"id":[{"id":"10.13039\/501100003785","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ethoxysanguinarine (ETSG), a benzophenanthridine alkaloid, exhibits diverse biological activities, including antibacterial, antifungal, anti\u2010inflammatory, antioxidant, and anti\u2010tumor effects. Despite these properties, limited research exists on ETSG in\u00a0vivo pharmacokinetics due to its poor solubility and low bioavailability. In this study, we developed a rapid and specific UPLC\u2010MS\/MS method for ETSG bioanalysis. Sample preparation involved one\u2010step protein precipitation using methanol and phellodendrine as an internal standard (IS). The Waters HSS T3 column (2.1\u2009*\u200950\u2009mm, 1.8\u2009\u03bcM) employed a gradient elution with mobile phases A (2\u2009mmol\/L ammonium formate aqueous solution\u2010formic acid [99.8:0.2, v\/v]) and B (methanol\u2010formic acid [99.8:0.2, v\/v]). Mass analysis via Waters Q\u2010mass spectrometer utilized positive scan mode and multiple reaction monitoring. ETSG and IS were detected at m\/z 332.0\u2009\u2192\u2009274.0 and 342.0\u2009\u2192\u2009177.0, respectively, within 7.0\u2009min. The method demonstrated excellent precision, accuracy, recovery, and stability, with a linear calibration curve (1.1\u2013560\u2009ng\/mL) and strong correlation coefficient (0.9984). Successful pharmacokinetic evaluation in Sprague\u2013Dawley rats included intravenous ETSG administration and intragastric ETSG nanoemulsion\/suspension. This method enables steroidal saponin analysis from ETSG in biological samples.<\/jats:p>","DOI":"10.1002\/bmc.6055","type":"journal-article","created":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T01:36:21Z","timestamp":1733276181000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of a UPLC\u2010MS\/MS Method for Bioanalysis of Ethoxysanguinarine and Its Application in Pharmacokinetic Study of Ethoxysanguinarine Nanoemulsion"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8889-8244","authenticated-orcid":false,"given":"Jianhui","family":"Luo","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Center for New Drug Research and Development Guangdong Pharmaceutical University  Guangzhou Guangdong China"},{"name":"Guangdong High Education Institutes Engineering Research Center of Modified\u2010Released Pharmaceutical Products, School of Pharmacy Guangdong Pharmaceutical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songshen","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Center for New Drug Research and Development Guangdong Pharmaceutical University  Guangzhou Guangdong China"},{"name":"Guangdong High Education Institutes Engineering Research Center of Modified\u2010Released Pharmaceutical Products, School of Pharmacy Guangdong Pharmaceutical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WenYang","family":"Song","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Center for New Drug Research and Development Guangdong Pharmaceutical University  Guangzhou Guangdong China"},{"name":"Guangdong High Education Institutes Engineering Research Center of Modified\u2010Released Pharmaceutical Products, School of Pharmacy Guangdong Pharmaceutical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongtong","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Center for New Drug Research and Development Guangdong Pharmaceutical University  Guangzhou Guangdong China"},{"name":"Guangdong High Education Institutes Engineering Research Center of Modified\u2010Released Pharmaceutical Products, School of Pharmacy Guangdong Pharmaceutical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Health Sciences Texas Southern University  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9593-605X","authenticated-orcid":false,"given":"Jiu","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Center for New Drug Research and Development Guangdong Pharmaceutical University  Guangzhou Guangdong China"},{"name":"Guangdong High Education Institutes Engineering Research Center of Modified\u2010Released Pharmaceutical Products, School of Pharmacy Guangdong Pharmaceutical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11051052"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5NP00073D"},{"key":"e_1_2_7_4_1","unstructured":"Food and Drug Administration. 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