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Cyclooxygenase 2 (COX\u20102) and lipoxygenase 5 (5\u2010LOX) were immobilized on magnetic nanoparticles for selective ligand\u2010extraction of these two enzymes present in <jats:styled-content style=\"fixed-case\"><jats:italic>C. axillaris<\/jats:italic><\/jats:styled-content>. Sixteen extracted components were identified and analyzed using ultraperformance liquid chromatography plus Q\u2010Exactive Orbitrap tandem mass spectrometry (UHPLC\u2010Q\u2010ExactiveOrbitrap\u2010MS\/MS), including flavonoids Curcumin and Epicatechin. The metabolization of the aforementioned 16 components in rats with acute blood stasis indicated a solid pharmacodynamic foundation. This method offers an effective approach for conducting basic research on the enrichment, analysis, and efficacy evaluation of active components in complex natural products.<\/jats:p>","DOI":"10.1002\/bmc.6060","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T06:01:40Z","timestamp":1736402500000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Noval Selective Ligand\u2010Extraction of Bioactive Components in Complex Natural Products Applying Immobilized Multi\u2010Target Magnetic Beads"],"prefix":"10.1002","volume":"39","author":[{"given":"Chen","family":"Wang","sequence":"first","affiliation":[{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Key Laboratory of Marine Bioresources and Environment Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueying","family":"Qin","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Key Laboratory of Marine Bioresources and Environment Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Key Laboratory of Marine Bioresources and Environment Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiping","family":"Wang","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Key Laboratory of Marine Bioresources and Environment Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Sun","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Key Laboratory of Marine Bioresources and Environment Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongsen","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiyu","family":"Li","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Key Laboratory of Marine Bioresources and Environment Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Dai","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Key Laboratory of Marine Bioresources and Environment Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianhong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Key Laboratory of Marine Bioresources and Environment Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7339-523X","authenticated-orcid":false,"given":"Xun","family":"Gao","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Key Laboratory of Marine Bioresources and Environment Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8463-1608","authenticated-orcid":false,"given":"Kunming","family":"Qin","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Key Laboratory of Marine Bioresources and Environment Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Lianyungang Affiliated Hospital of Nanjing University of Chinese Medicine  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis\u20102011\u2010200726"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","unstructured":"Azam A. 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