{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:40:19Z","timestamp":1777390819755,"version":"3.51.4"},"reference-count":5,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The fixed\u2010dose combination of pregabalin and duloxetine hydrochloride is mainly used in the treatment of neuropathic pain. Sensitive determination of drugs like pregabalin is challenging by chromatographic methods using UV detection due to their nonchromophoric nature. This study presents the development of a validated high\u2010performance thin\u2010layer chromatography (HPTLC) method for the simultaneous determination pregabalin and duloxetine hydrochloride in formulation. Because pregabalin has a weak chromophoric system, it was visualized using a derivatization reagent containing ninhydrin. Optimum separation of pregabalin and duloxetine hydrochloride was achieved on silica gel 60 F<jats:sub>254<\/jats:sub> plates using methanol:dichloromethane:acetone:ammonia (8:1:1:0.2, v\/v\/v\/v). Good resolution was achieved with R<jats:sub>f<\/jats:sub> values of 0.34\u2009\u00b1\u20090.02 and 0.52\u2009\u00b1\u20090.02 for duloxetine hydrochloride and pregabalin, respectively. The method was validated as per the ICH Q2(R1) guidelines. Linearity range was found between 200 and 450\u2009ng\/band for duloxetine and 500 and 1125\u2009ng\/band for pregabalin. Low %RSD values shows that the developed method is precise. Greenness assessment of the developed method was evaluated using three different assessment tools, namely, Analytical Eco\u2010Scale, GAPI, and AGREE This is the first validated derivatization\u2010based HPTLC method for simultaneous analysis of duloxetine hydrochloride and pregabalin.<\/jats:p>","DOI":"10.1002\/bmc.6078","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T04:31:37Z","timestamp":1738902697000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Derivatization\u2010Based Densitometric Approach Along With Greenness Assessment for Simultaneous Evaluation of Duloxetine Hydrochloride and Pregabalin: An Application to Pharmaceutical Analysis"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0978-3120","authenticated-orcid":false,"given":"Susheel\u00a0John","family":"Varghese","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis, College of Pharmacy Sri Ramakrishna Institute of Paramedical Sciences (Affiliated to Tamil Nadu Dr M.G.R. Medical University)  Coimbatore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karthikeyan","family":"Venkatesan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, College of Pharmacy Sri Ramakrishna Institute of Paramedical Sciences (Affiliated to Tamil Nadu Dr M.G.R. Medical University)  Coimbatore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thengungal\u00a0Kochupappy","family":"Ravi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, College of Pharmacy Sri Ramakrishna Institute of Paramedical Sciences (Affiliated to Tamil Nadu Dr M.G.R. Medical University)  Coimbatore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115734129321062240906070516"},{"key":"e_1_2_6_3_1","unstructured":"ICH Q2 (R1).2005. \u201cValidation of Analytical Procedures: Text and Methodology.\u201d"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.13040\/IJPSR.0975\u20108232"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474\u20104422(10)70143\u20105"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2013.05.043"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.6078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:12:57Z","timestamp":1760040777000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.6078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,7]]},"references-count":5,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/bmc.6078"],"URL":"https:\/\/doi.org\/10.1002\/bmc.6078","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,7]]},"assertion":[{"value":"2024-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e6078"}}