{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T20:08:03Z","timestamp":1767211683490,"version":"build-2065373602"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T00:00:00Z","timestamp":1736553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012659","name":"Foundation for Innovative Research Groups of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32121004"],"award-info":[{"award-number":["32121004"]}],"id":[{"id":"10.13039\/501100012659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Praziquantel (PZQ) is the most effective treatment for schistosomiasis, commonly administered as a racemic mixture of the two enantiomers. Despite many reports on the pharmacokinetics of PZQ, the stereoselective pharmacokinetics of PZQ and its major metabolite 4\u2010hydroxypraziquantel (4\u2010OH\u2010PZQ) remain poorly understood in goats. In this study, the chiral LC\u2010MS\/MS method was further optimized for separating and quantifying PZQ, <jats:italic>trans<\/jats:italic>\u20104\u2010OH\u2010PZQ, and <jats:italic>cis<\/jats:italic>\u20104\u2010OH\u2010PZQ and their enantiomers and then applied for the molecular pharmacokinetics of three analytes in black goat plasma. The findings showed that PZQ was rapidly absorbed and metabolized to 4\u2010OH\u2010PZQ. The C<jats:sub>max<\/jats:sub> of <jats:italic>trans<\/jats:italic>\u20104\u2010OH\u2010PZQ was about 3 times and 6 times higher than those of <jats:italic>cis<\/jats:italic>\u20104\u2010OH\u2010PZQ and PZQ, respectively. The stereoselectivity of the PZQ and <jats:italic>cis<\/jats:italic>\u20104\u2010OH\u2010PZQ enantiomers was insignificant in black goat plasma (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05), whereas the <jats:italic>trans<\/jats:italic>\u20104\u2010OH\u2010PZQ enantiomers exhibited obvious stereoselectivity (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). The C<jats:sub>max<\/jats:sub> of S\u2010trans\u20104\u2010OH\u2010PZQ were ~3.1 times higher than that of R\u2010<jats:italic>trans<\/jats:italic>\u20104\u2010OH\u2010PZQ. Further computer simulations indicated that these differences in the stereoselectivity might mainly stem from the different binding energies of the corresponding R\u2010 and S\u2010enantiomers of the target analytes to black goat plasma albumin. It has guiding significance for the research on the stereoselectivity of chiral veterinary drugs and their precision medication.<\/jats:p>","DOI":"10.1002\/bmc.6082","type":"journal-article","created":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T07:41:03Z","timestamp":1736581263000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["LC\u2010MS\/MS Analyzing Praziquantel and 4\u2010Hydroxypraziquantel Enantiomers in Black Goat Plasma and Mechanism of Stereoselective Pharmacokinetics"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1787-0139","authenticated-orcid":false,"given":"Xiaoxi","family":"Du","sequence":"first","affiliation":[{"name":"Guangdong Provincial key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation South China Agricultural University  Guangzhou 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