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A selective and sensitive method has been developed and validated for the quantitation of TXM in human plasma using high performance liquid chromatography coupled with tandem mass spectrometry (HPLC\u2010MS\/MS). Tranexamic acid\u2010D2 (TXM\u2010D2) was used as internal standard (ISTD) to minimize the errors in TXM quantification. TXM quantification was performed with positive polarity mode using a Shimadzu high performance liquid chromatography coupled with AB\u2010SCIEX API\u20104000 tandem mass spectrometer. A Zorbax Eclipse C18 (150\u2009\u00d7\u20094.6\u2009mm, 5\u2009\u03bc) column was used for the TXM quantification, 8\u2010mM ammonium formate buffer with 0.1% formic acid ionization enhancer was used as a mobile phase\u2010A, and acetonitrile was used as a mobile phase\u2010B. (38:62) v\/v portion of mobile phases A and B selected to elute the TXM and TXM\u2010D2 with the flow rate of 0.8\u2009mL\/min. An electrospray ionization (ESI) technique was selected for detection of TXM in the human plasma. Linearity was assessed in the concentration range from 75 to 15,000\u2009ng\/mL by using least squares of weighting factor linear regression (1\/X<jats:sup>2<\/jats:sup>).<\/jats:p>","DOI":"10.1002\/bmc.6086","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T04:27:47Z","timestamp":1738902467000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Bioanalytical Tandem Mass Spectrometry Method for Precise Measurement of Tranexamic Acid in Human Plasma With Unveiling Methodological Advancement: Green Assessment With Advanced Metrics"],"prefix":"10.1002","volume":"39","author":[{"given":"Anil","family":"Kollapareddy","sequence":"first","affiliation":[{"name":"Department of Chemistry Koneru Lakshmaiah Education Foundation  Hyderabad Telangana 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