{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T09:45:07Z","timestamp":1784713507444,"version":"3.55.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Pharmacokinetic studies can provide a method for optimizing preparations. In this study, three most abundant compounds from <jats:italic>Fritillaria ussuriensis<\/jats:italic> bulb were isolated, and a selective, sensitive new analytical method was developed. This method aims for the simultaneous determination of peimisine\u20103\u2010O\u2010\u03b2\u2010D\u2010glucopyranoside (<jats:bold>1<\/jats:bold>), karelinine (<jats:bold>2<\/jats:bold>), and peimisine (<jats:bold>3<\/jats:bold>) in rat plasma after oral administration of <jats:italic>F. ussuriensis<\/jats:italic> bulb powder (FUP), total alkaloid extract (FUE), and its nanodispersion preparation (FUN) using UHPLC\u2013MS\/MS. The chromatographic separation was performed on the UHPLC Waters BEH C18 column. The pharmacokinetic results showed that among three groups, <jats:bold>1<\/jats:bold> and <jats:bold>3<\/jats:bold> exhibited slower absorption and elimination processes when rats were orally administered with FUP and FUE. Steroidal alkaloids <jats:bold>1<\/jats:bold>\u2009~\u2009<jats:bold>3<\/jats:bold> from FUN reached peak concentrations within 3\u2009h. Compared with FUE and FUP, the absorption and elimination rates were accelerated, and the area under the curve (AUC) significantly increased. In this study, the pharmacokinetic characteristics of <jats:bold>1<\/jats:bold>\u2009~\u2009<jats:bold>2<\/jats:bold> were first detected and calculated parameters in rat plasma when orally administered in different dosage forms. And a new nanodispersion preparation, FUN, has been developed. FUN has better absorption efficiency and higher bioavailability compared with traditional powder oral administration.<\/jats:p>","DOI":"10.1002\/bmc.6087","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T04:20:37Z","timestamp":1738902037000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Simultaneous Determination and Pharmacokinetics of Three Steroidal Alkaloids in Rat Plasma by UHPLC\u2013MS After Oral Administration of Three Different Preparations of <i>Fritillaria ussuriensis<\/i> Maxim Bulb"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4017-1275","authenticated-orcid":false,"given":"Haibo","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education Heilongjiang University of Chinese Medicine  Harbin 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