{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T11:54:16Z","timestamp":1762430056508,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,2,7]],"date-time":"2006-02-07T00:00:00Z","timestamp":1139270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The enzyme catechol\u2010<jats:italic>O\u2010<\/jats:italic>methyltransferase (COMT) plays an important role in the metabolism of catechol estrogens and degradation of the catecholamine neurotransmitters, such as epinephrine. Several analytical methods, mainly high\u2010performance liquid chromatography with electrochemical amperometric detection, have been reported for the analysis of catecholamines and their metabolites in biological fluids. In this paper we report the relevance of controlling temperature in calibration procedures of metanephrine, an <jats:italic>O<\/jats:italic>\u2010methylated product of catechol\u2010<jats:italic>O\u2010<\/jats:italic>methyltransferase, using epinephrine as substrate. The results at higher temperatures show shorter retention times of metanephrine, no undue band\u2010broadening and increased electro signals. This study also showed that, despite different temperatures leading to similarly specific activities of recombinant human COMT as expected, there are additional advantages in flow analytical methods where good sensitivity, efficiency and selectivity is required, mainly in tissues with low levels of COMT activity. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.623","type":"journal-article","created":{"date-parts":[[2006,2,9]],"date-time":"2006-02-09T14:52:00Z","timestamp":1139496720000},"page":"937-944","source":"Crossref","is-referenced-by-count":14,"title":["The effect of temperature on the analysis of metanephrine for catechol\u2010O\u2010methyltransferase activity assay by HPLC with electrochemical detection"],"prefix":"10.1002","volume":"20","author":[{"given":"L. A.","family":"Passarinha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Bonif\u00e1cio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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