{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T01:42:58Z","timestamp":1773625378340,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,5,16]],"date-time":"2001-05-16T00:00:00Z","timestamp":989971200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2001,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vancomycin is an amphoteric, glycopeptide, macrocyclic antibiotic. When attached to 5\u2005\u00b5spherical silica gel, vancomycin proved to be an effective chromatographic chiral stationary phase that could be used in the reversed\u2010phase mode. In this study, a bonded vancomycin chiral stationary phase (Chirobiotic V\u2122) was investigated for the chiral liquid chromatography analysis of ketoprofen and flurbiprofen. The selectivity factor (\u03b1) and the chiral resolution factor (<jats:italic>R<\/jats:italic><jats:sub>S<\/jats:sub>) of Chirobiotic V\u2122 were evaluated first as a function of the buffer pH and molarity, and second as a function of organic modifier type and composition of the mobile phase. Four organic modifiers (tetrahydrofuran, 2\u2010propanol, 1,4\u2010dioxane and methanol) have been tested for their selectivity. Optimized conditions using 20% of tetrahydrofuran in ammonium nitrate (100\u2005m<jats:sc>M<\/jats:sc>, pH 5) were selected for the enantioseparation of flurbiprofen and ketoprofen from their racemic forms. At pH 5, these acidic compounds are almost negatively charged, while the chiral selector possesses a positive charge allowing it to interact electrostatistically with the analytes. Using these chromatographic conditions, the column stability was excellent over several months of experiments. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.65","type":"journal-article","created":{"date-parts":[[2002,9,10]],"date-time":"2002-09-10T19:33:12Z","timestamp":1031686392000},"page":"217-222","source":"Crossref","is-referenced-by-count":43,"title":["Chiral resolution of flurbiprofen and ketoprofen enantiomers by HPLC on a glycopeptide\u2010type column chiral stationary phase"],"prefix":"10.1002","volume":"15","author":[{"given":"F.","family":"P\u00e9hourcq","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Jarry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Bannwarth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,5,16]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-0801(199705)11:3<137::AID-BMC648>3.0.CO;2-T"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(97)00161-1"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(94)00875-A"},{"key":"e_1_2_1_5_1","unstructured":"ASTEC (Advanced Separation Technologies Inc.).Chirobiotic\u2122 handbook. A guide to using macrocyclic glycopeptide bonded phases for chiral LC separations 3rd edn technical document 1999."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.1995.tb05783.x"},{"key":"e_1_2_1_7_1","volume-title":"Disposition of Toxic Drugs and Chemicals in Man","author":"Baselt RA","year":"1996"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600780806"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-0801(199811\/12)12:6<309::AID-BMC763>3.0.CO;2-K"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(96)00402-1"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826079408013986"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-636X(1998)10:7<627::AID-CHIR10>3.0.CO;2-1"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(92)80492-9"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1552-4604.1996.tb05041.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(93)80004-N"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-198409040-00007"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)83780-9"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(91)80478-U"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-0801(199703)11:2<83::AID-BMC646>3.0.CO;2-L"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826079508013739"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00361-1"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(93)80818-S"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(94)E0012-P"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826079508013972"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826079808000492"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-198530040-00003"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.65","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.65","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T23:29:09Z","timestamp":1760052549000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.65"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,5]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,5]]}},"alternative-id":["10.1002\/bmc.65"],"URL":"https:\/\/doi.org\/10.1002\/bmc.65","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,5]]}}}