{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T13:13:12Z","timestamp":1771074792462,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A precise and stability\u2010indicating high\u2010performance liquid chromatographic (HPLC) method was developed and validated for the simultaneous estimation of lobeglitazone sulfate (0.5\u2009mg) and metformin hydrochloride (500\u2009mg) in pharmaceutical formulations. This method effectively addresses the analytical challenge posed by the significantly different dosage strengths and contrasting polarities of lobeglitazone (water insoluble) and metformin (water soluble). Utilizing a Phenomenex Strong Cation Exchange (250\u2009mm\u2009\u00d7\u20094.6\u2009mm, 10\u2009\u03bcm) column, the method employed a mobile phase of ammonium dihydrogen phosphate (pH\u20093.0) and a premix of methanol and acetonitrile (80:20), delivered at a flow rate of 1.0\u2009mL\/min. The PDA detector was set at 251\u2009nm to monitor eluents, and the column temperature was maintained at 30\u00b0C for optimal separation. The method was validated as per ICH guidelines, covering specificity, linearity, accuracy, precision, LOD, LOQ, solution stability, and robustness. Forced degradation studies under various stress conditions (acidic, basic, oxidative, photolytic, and thermal) revealed no significant degradation products. The method was also assessed for greenness using a AGREE tool, achieving a score of 0.68, demonstrating its eco\u2010friendly nature. Overall, this method is suitable for the routine analysis of these drugs in pharmaceutical formulations.<\/jats:p>","DOI":"10.1002\/bmc.70013","type":"journal-article","created":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T00:56:02Z","timestamp":1739321762000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bridging the Gap: Eco\u2010Friendly HPLC Analysis of Metformin and Lobeglitazone Sulfate Despite Polarity and Dosage Differences"],"prefix":"10.1002","volume":"39","author":[{"given":"Honey","family":"Kevat","sequence":"first","affiliation":[{"name":"School of Pharmacy Gujarat Technological University  Gandhinagar Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayushi","family":"Prajapati","sequence":"additional","affiliation":[{"name":"School of Pharmacy Gujarat Technological University  Gandhinagar Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiran","family":"Parmar","sequence":"additional","affiliation":[{"name":"School of Pharmacy Gujarat Technological University  Gandhinagar Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhumika","family":"Maheriya","sequence":"additional","affiliation":[{"name":"School of Pharmacy Gujarat Technological University  Gandhinagar Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hardi","family":"Joshi","sequence":"additional","affiliation":[{"name":"School of Pharmacy Gujarat Technological University  Gandhinagar Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jigna","family":"Vadalia","sequence":"additional","affiliation":[{"name":"School of Pharmacy Gujarat Technological University  Gandhinagar Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5603-2330","authenticated-orcid":false,"given":"Kashyap","family":"Thummar","sequence":"additional","affiliation":[{"name":"School of Pharmacy Gujarat Technological University  Gandhinagar Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.07.009"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691\u2010199906000\u201000015"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115734129303749240607083542"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.9884"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2018.06.007"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43094\u2010024\u201000734\u2010x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpdd.625"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43094\u2010020\u201000079\u20101"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2009.06.003"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202000937"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5155"},{"key":"e_1_2_11_13_1","unstructured":"NCBI.2023. \u201cNational Center for Biotechnology Information.\u201d PubChem Compound Summary for CID 9826451 Lobeglitazone. Retrieved fromhttps:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/Lobeglitazone."},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11094-021-02546-2"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.202400059"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2024.111211"},{"key":"e_1_2_11_17_1","unstructured":"Susmita Roy.2022. \u201cGlenmark Gets CDSCO Panel Nod To Manufacture Market Lobeglitazone Metfromin Antidiabetic FDC.\u201d Retrieved fromhttps:\/\/medicaldialogues.in\/news\/industry\/pharma\/glenmark\u2010gets\u2010cdsco\u2010panel\u2010nod\u2010to\u2010manufacturemarket\u2010lobeglitazone\u2010metformin\u2010antidiabetic\u2010fdc\u2010101532#:~:text=marketing%20the%E2%80%A6New%20Delhi%3A%20Highlighting%20the%20clinical%20trial%20results%20that%20demonstrate%20the Control%20Organization%20(CDSCO)%20has%20granted."},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/28378083.2024.2409233"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3797\/scipharm.1411\u201004"},{"key":"e_1_2_11_20_1","volume-title":"Essentials of Medical Pharmacology","author":"Tripathi K.","year":"2018"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5384"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104347(98)00305\u20103"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.4103\/0250\u2010474x.41455"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:12:41Z","timestamp":1760040761000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,11]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/bmc.70013"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70013","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,11]]},"assertion":[{"value":"2024-10-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70013"}}