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It is based on the analytes reduction by tris (2\u2010carboxyethyl) phosphine and derivatization with N\u2010ethylmaleimide, with subsequent ultrafiltration of the sample. Separation was performed using 0.1\u2010M phosphate Na \u0440\u041d 2.48 with 1% Ficoll\u2010400 and 10% methanol. The in\u2010capillary concentration of the analytes was achieved with a pH gradient created via the preinjection of triethanolamine. The absorbance signal was detected at 200\u2009nm. The total analysis time was 16\u2009min (using a silica capillary [50\u2009\u03bcm i.d.; total length 42\u2009cm] at a 20\u2010kV voltage). The limits of detection varied from 1.3\u2009\u03bcM (glutathione) to 5.4\u2009\u03bcM (cysteine). Within\u2010run and between\u2010run precision (CV) was &lt;\u20097%; reduction of analytes ranging from 91% to 106%, were obtained. The approach was validated on control samples of children's blood plasma. The simplicity of the method and the availability of reagents make the presented approach most suitable for use on standard <jats:sc>CE<\/jats:sc> systems.<\/jats:p>","DOI":"10.1002\/bmc.70017","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T04:18:27Z","timestamp":1738901907000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Determination of Main Aminothiols in Blood Plasma Using Capillary Electrophoresis With UV Detection"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-5238-8122","authenticated-orcid":false,"given":"Polina\u00a0Alexandrovna","family":"Pudova","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Pathophysiology Federal State Budgetary Scientific Institution Institute of General Pathology and Pathophysiology  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander\u00a0Vladimirovich","family":"Ivanov","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Pathophysiology Federal State Budgetary Scientific Institution Institute of General Pathology and Pathophysiology  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail\u00a0Aleksandrovich","family":"Popov","sequence":"additional","affiliation":[{"name":"Moscow Regional Research and Clinical Institute n.a. 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