{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:05:00Z","timestamp":1760141100746,"version":"build-2065373602"},"reference-count":56,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Ixeris sonchifolia<\/jats:italic> (IS) has been demonstrated to have beneficial effects on clearing heat and detoxifying, promoting blood circulation and removing blood stasis. However, the protective effects of active fractions and the underlying mechanisms of IS against toxic heat and blood stasis syndrome (THBSS) remain unclear. This study aimed to investigate this. In this study, the protective effects of 30%, 50%, and 70% ethanol fractions of IS on rats with THBSS were evaluated by observing myocardial pathology, analyzing blood rheology and blood routine indices, and the general conditions of the rats. Comprehensive metabolomic analysis of plasma and myocardium samples was conducted using ultra\u2010high\u2010performance liquid chromatography linear ion trap quadrupole orbitrap mass spectrometry (UHPLC\u2010LTQ\u2010Orbitrap MS\/MS). The results showed that IS\u201030% had a significant protective effect on rats with THBSS, and it could play a protective role by modulating 35 differential metabolites associated with key metabolic pathways, including glycerophospholipid metabolism, sphingosine metabolism, alpha\u2010linolenic acid metabolism, and nicotinate and nicotinamide metabolism. Analysis of different metabolites and pathways showed that they are closely associated with inflammatory responses; therefore, it is hypothesized that IS\u201030% may protect against THBSS by exerting an anti\u2010inflammatory effect. This study provides valuable data supporting the druggability of IS\u201030% and offers new insights into its potential therapeutic applications.<\/jats:p>","DOI":"10.1002\/bmc.70042","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T18:50:46Z","timestamp":1741805446000},"page":"1-15","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective Effects and Mechanisms of Active Fractions of <i>Ixeris sonchifolia<\/i> on Rats With Toxic Heat and Blood Stasis Syndrome Revealed by Pharmacodynamics and Metabonomics"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9145-649X","authenticated-orcid":false,"given":"Zhaowei","family":"Liu","sequence":"first","affiliation":[{"name":"Beijing Research Institute of Chinese Medicine Beijing University of Chinese Medicine  Beijing China"},{"name":"School of Chinese Pharmacy Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0022-9986","authenticated-orcid":false,"given":"Yuanyuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Research Institute of Chinese Medicine Beijing University of Chinese Medicine  Beijing China"},{"name":"School of Chinese Pharmacy Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiran","family":"Ren","sequence":"additional","affiliation":[{"name":"Beijing Research Institute of Chinese Medicine Beijing University of Chinese Medicine  Beijing China"},{"name":"School of Chinese Pharmacy Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifei","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Research Institute of Chinese Medicine Beijing University of Chinese Medicine  Beijing China"},{"name":"School of Chinese Pharmacy Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenqing","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Research Institute of Chinese Medicine Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingxuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Medicilon Inc.  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Research Institute of Chinese Medicine Beijing University of Chinese Medicine  Beijing China"},{"name":"Beijing Key Laboratory for Production Process Control and Quality Evaluation of Traditional Chinese Medicine Beijing Municipal Science &amp; Technology Commission  Beijing China"},{"name":"Engineering Research Center for Pharmaceutics of Chinese Materia Medica and New Drug Development Ministry of Education  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,12]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_8_2_1","DOI":"10.1038\/nature06613"},{"doi-asserted-by":"publisher","key":"e_1_2_8_3_1","DOI":"10.1186\/1471-2164-13-334"},{"doi-asserted-by":"publisher","key":"e_1_2_8_4_1","DOI":"10.1016\/B978-0-12-815776-3.00001-2"},{"doi-asserted-by":"publisher","key":"e_1_2_8_5_1","DOI":"10.1002\/jms.3503"},{"doi-asserted-by":"publisher","key":"e_1_2_8_6_1","DOI":"10.1002\/bmc.5658"},{"doi-asserted-by":"publisher","key":"e_1_2_8_7_1","DOI":"10.1111\/jcmm.14418"},{"doi-asserted-by":"publisher","key":"e_1_2_8_8_1","DOI":"10.1016\/j.jacc.2022.04.060"},{"doi-asserted-by":"publisher","key":"e_1_2_8_9_1","DOI":"10.1161\/01.ATV.15.11.1987"},{"doi-asserted-by":"publisher","key":"e_1_2_8_10_1","DOI":"10.1161\/ATVBAHA.111.241836"},{"key":"e_1_2_8_11_1","first-page":"413","article-title":"Construction and Application of Heat Toxicity Doctrine in Cardiac Diseases","volume":"28","author":"Ding W. S.","year":"2004","journal-title":"Shandong Journal of Traditional Chinese Medicine"},{"doi-asserted-by":"publisher","key":"e_1_2_8_12_1","DOI":"10.1016\/j.bbrc.2023.01.011"},{"doi-asserted-by":"publisher","key":"e_1_2_8_13_1","DOI":"10.1055\/s-2003-45152"},{"key":"e_1_2_8_14_1","first-page":"31","article-title":"Pharmacological Effects of Ixeris sonchifolia on the Cardiovascular System","volume":"3","author":"Feng Y. S.","year":"1979","journal-title":"Zhongcaoyao"},{"doi-asserted-by":"publisher","key":"e_1_2_8_15_1","DOI":"10.1096\/fj.201800221R"},{"doi-asserted-by":"publisher","key":"e_1_2_8_16_1","DOI":"10.3390\/nu14204414"},{"doi-asserted-by":"publisher","key":"e_1_2_8_17_1","DOI":"10.2298\/GENSR1401075J"},{"doi-asserted-by":"publisher","key":"e_1_2_8_18_1","DOI":"10.3390\/ijms21124501"},{"doi-asserted-by":"publisher","key":"e_1_2_8_19_1","DOI":"10.1016\/j.biopha.2020.109869"},{"key":"e_1_2_8_20_1","first-page":"1946","article-title":"Heat\u2010Toxicity and Injury of Collateral Is the Basic Pathology of the Heat\u2010Toxicity and Blood Stasis Syndrome","volume":"25","author":"Li M.","year":"2010","journal-title":"Zhonghua Zhongyiyao Zazhi"},{"key":"e_1_2_8_21_1","first-page":"2124","article-title":"Establishment of an Animal Model of Heat Toxicity\u2010Induced Thrombosis","volume":"33","author":"Liang A. H.","year":"2008","journal-title":"Zhongguo Zhongyao Zazhi"},{"doi-asserted-by":"publisher","key":"e_1_2_8_22_1","DOI":"10.1016\/j.jchromb.2023.123626"},{"key":"e_1_2_8_23_1","first-page":"1293","article-title":"Research Progress on Correlation Among White Blood Cell Counts, Diabetes and Its Complications","volume":"15","author":"Liu L.","year":"2012","journal-title":"Zhongguo Quanke Yixue"},{"key":"e_1_2_8_24_1","first-page":"955","article-title":"UHPLC\u2010LTQ\u2010Orbitrap\u2010Based Metabolomics Coupled With Metabolomics Pathway Analysis Method for Exploring the Protection Mechanism of Kudiezi Injection in a Rat Anti\u2010Ischemic Cerebral Reperfusion Damage Model","volume":"15","author":"Liu S. Y.","year":"2017","journal-title":"Zhongguo Zhongyao Zazhi"},{"doi-asserted-by":"publisher","key":"e_1_2_8_25_1","DOI":"10.3748\/wjg.v19.i22.3423"},{"key":"e_1_2_8_26_1","first-page":"67","article-title":"Effects of Kudiezi Injection Pretreatment on Neural Function and Hippocampal Neurons in Rats With Blood Stasis and Toxin Syndrome of Cerebral Ischemia\u2010Reperfusion Injury After Acute Ischemic Stroke","volume":"29","author":"Ma R. X.","year":"2021","journal-title":"Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease"},{"doi-asserted-by":"publisher","key":"e_1_2_8_27_1","DOI":"10.1016\/j.clnu.2016.10.012"},{"doi-asserted-by":"publisher","key":"e_1_2_8_28_1","DOI":"10.1016\/j.bbadis.2008.05.002"},{"key":"e_1_2_8_29_1","first-page":"67","article-title":"Chlorogenic Acid (CGA): A Pharmacological Review and Call for Further Research","volume":"97","author":"Naveed M.","year":"2018","journal-title":"Cardiovascular Research"},{"doi-asserted-by":"publisher","key":"e_1_2_8_30_1","DOI":"10.1093\/cvr\/cvaa275"},{"doi-asserted-by":"publisher","key":"e_1_2_8_31_1","DOI":"10.2217\/bmm-2018-0050"},{"doi-asserted-by":"publisher","key":"e_1_2_8_32_1","DOI":"10.1007\/s12061-021-09382-7"},{"doi-asserted-by":"publisher","key":"e_1_2_8_33_1","DOI":"10.1016\/j.jri.2016.06.001"},{"doi-asserted-by":"publisher","key":"e_1_2_8_34_1","DOI":"10.1371\/journal.pone.0027683"},{"key":"e_1_2_8_35_1","first-page":"209","article-title":"The Relationship of HCT Between Age and ESR","volume":"38","author":"Tian P. P.","year":"2017","journal-title":"Guoji Jianyan Yixue Zazhi"},{"doi-asserted-by":"publisher","key":"e_1_2_8_36_1","DOI":"10.3390\/molecules24050964"},{"doi-asserted-by":"publisher","key":"e_1_2_8_37_1","DOI":"10.1055\/s-2007-1000390"},{"doi-asserted-by":"publisher","key":"e_1_2_8_38_1","DOI":"10.3390\/nu10050604"},{"doi-asserted-by":"publisher","key":"e_1_2_8_39_1","DOI":"10.1007\/s10787-019-00612-6"},{"doi-asserted-by":"publisher","key":"e_1_2_8_40_1","DOI":"10.3390\/molecules23061315"},{"key":"e_1_2_8_41_1","first-page":"11","article-title":"Hemorheology and Blood Stasis","volume":"1","author":"Wang J. M.","year":"1989","journal-title":"Journal of Nanjing University of Traditional Chinese Medicine"},{"doi-asserted-by":"publisher","key":"e_1_2_8_42_1","DOI":"10.1007\/s10753-019-01099-7"},{"doi-asserted-by":"publisher","key":"e_1_2_8_43_1","DOI":"10.1016\/j.jchromb.2018.12.017"},{"key":"e_1_2_8_44_1","first-page":"3472","article-title":"Exploration and Analysis on Toxic Heat and Blood Stasis Syndrome","volume":"28","author":"Xie G. Q.","year":"2013","journal-title":"Zhonghua Zhongyiyao Zazhi"},{"doi-asserted-by":"publisher","key":"e_1_2_8_45_1","DOI":"10.1039\/D0RA07038F"},{"doi-asserted-by":"publisher","key":"e_1_2_8_46_1","DOI":"10.3389\/fphar.2022.842839"},{"doi-asserted-by":"publisher","key":"e_1_2_8_47_1","DOI":"10.1016\/j.jep.2022.115943"},{"key":"e_1_2_8_48_1","first-page":"2168","article-title":"Research Progress of Correlation Between Toxic\u2010Heat\u2010Blood\u2010Stasis Syndrome and Inflammation","volume":"28","author":"Yang W.","year":"2010","journal-title":"Chinese Journal of Integrative Medicine"},{"doi-asserted-by":"publisher","key":"e_1_2_8_49_1","DOI":"10.3389\/fnagi.2021.691230"},{"doi-asserted-by":"publisher","key":"e_1_2_8_50_1","DOI":"10.1016\/j.bios.2015.02.019"},{"doi-asserted-by":"publisher","key":"e_1_2_8_51_1","DOI":"10.1016\/j.fct.2013.05.058"},{"doi-asserted-by":"publisher","key":"e_1_2_8_52_1","DOI":"10.1080\/10286020902727520"},{"key":"e_1_2_8_53_1","first-page":"586","article-title":"Research on Kudiezi Injection Treating for Guizhou Minipig With Hepatic Fibrosis","volume":"21","author":"Zhao J. H.","year":"2010","journal-title":"Shizhen Guoyi Guoyao"},{"key":"e_1_2_8_54_1","first-page":"165","article-title":"Effect of Sowthistle\u2010Leaf Ixeris Seedling Saponins on Blood Lipid Metabolism and Antioxidative Activity in Experimental Hyperlipidemia Rats","volume":"3","author":"Zhao Y. W.","year":"2005","journal-title":"Journal of Logistics University of CAPF"},{"key":"e_1_2_8_55_1","first-page":"482","article-title":"Chemical Compositions in Different Elution Parts of Ixeris sonchifolia (Bunge) Hance Alcohol Extract by UHPLC\u2010LTQ\u2010Orbitrap MSn","volume":"20","author":"Zhao Y. Y.","year":"2022","journal-title":"Zhongnan Yaoxue"},{"key":"e_1_2_8_56_1","first-page":"73","article-title":"Study on Potential Biomarkers and Metabolic Pathways of Rats in Different Progressive Stages of Coronary Heart Disease With Blood Stasis Syndrome Based on Metabonomics Technology","volume":"29","author":"Zhou M. L.","year":"2022","journal-title":"Zhongguo Zhongyiyao Xinxi Zazhi"},{"doi-asserted-by":"publisher","key":"e_1_2_8_57_1","DOI":"10.1016\/j.jep.2020.112618"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70042","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:07:16Z","timestamp":1760054836000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,12]]},"references-count":56,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/bmc.70042"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70042","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2025,3,12]]},"assertion":[{"value":"2024-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}