{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T16:15:53Z","timestamp":1760890553810,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["SL2022A04J01943"],"award-info":[{"award-number":["SL2022A04J01943"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A20535","82025034","82274001","82304457","82304603"],"award-info":[{"award-number":["U23A20535","82025034","82274001","82304457","82304603"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Compound 13b, a newly identified anthraquinone derivative, has demonstrated potent efficacy against the Zika virus. To explore the bioavailability and pharmacokinetic properties of compound 13b, a robust and sensitive HPLC\u2010MS\/MS method was established and verified to determine its plasma concentration in rats. Sample preparation involved protein precipitation using acetonitrile with testosterone as an internal standard. A Phenomenex Kinetex XB\u2010C18 column (2.1\u2009\u00d7\u2009100\u2009mm, 2.6\u2009\u03bcm) was utilized for sample separation with gradient elution. The mobile phase, consisting of water and acetonitrile, was dispensed at a flow rate of 1\u2009mL\/min. The multiple reaction monitoring mode (MRM) approach was used to detect compound 13b and testosterone, characterized by their respective ionization transitions: m\/z 410.2\u2009\u2192\u200991.1 and m\/z 289.2\u2009\u2192\u2009109.2. The method demonstrated superior linearity within rat plasma samples, ranging from 2 to 400\u2009ng\/mL, and met all FDA guidelines for bioanalytical method validation. The pharmacokinetic properties were calculated by non\u2010compartmental approach following administration of compound 13b at 14\u2009mg\/kg in rats, and the absolute oral bioavailability was found to be 15.45%. Hence, a highly sensitive and rapid HPLC\u2010MS\/MS method for measuring plasma concentration of compound 13b in rats has been successfully developed, rigorously validated, and subsequently utilized in a bioavailability and pharmacokinetic study.<\/jats:p>","DOI":"10.1002\/bmc.70044","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T06:33:25Z","timestamp":1741761205000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development and Validation of a HPLC\u2010MS\/MS Method for the Determination of Compound 13b in Rat Plasma and Its Application to a Pharmacokinetic Study"],"prefix":"10.1002","volume":"39","author":[{"given":"Guofang","family":"Bi","sequence":"first","affiliation":[{"name":"NMPA Key Laboratory for Research and Evaluation of Drug Metabolism &amp; Guangdong Provincial Key Laboratory of New Drug Screening &amp; Guangdong\u2010Hongkong\u2010Macao Joint Laboratory for New Drug Screening, School of Pharmaceutical Sciences Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingqing","family":"Yu","sequence":"additional","affiliation":[{"name":"NMPA Key Laboratory for Research and Evaluation of Drug Metabolism &amp; 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