{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:26:44Z","timestamp":1780471604309,"version":"3.54.1"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100017577","name":"Basic Public Welfare Research Program of Zhejiang Province","doi-asserted-by":"publisher","award":["LGC22H300003"],"award-info":[{"award-number":["LGC22H300003"]}],"id":[{"id":"10.13039\/501100017577","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A novel and rapid RP\u2010HPLC coupled with nano quantity analyte detector (NQAD) method was developed for determination of ethambutol hydrochloride and (+)2\u2010aminobutanol in drug products. NQAD is an aerosol\u2010based detector that can be used for the direct detection of the substance lacking ultraviolet chromophores. The two highly polar analytes were separated on a C<jats:sub>18<\/jats:sub> column with mobile phase consists of trifluoroacetic acid solution and methanol and detected with NQAD. A thorough investigation was conducted into the experimental and instrumental parameters, including the composition and ratio of mobile phase, the flow\u2010rate of mobile phase, and nebulizer and evaporator temperature, to achieve the highest sensitivity of analytes. The method was validated as per ICH guidelines and proved to be specific, accurate, precise, linear, sensitive, and robust. The method showed adequate performance, with excellent sensitivity for limits of detection (LODs) of 5.6 and 9.8\u2009ng for ethambutol and (+)2\u2010aminobutanol (Impurity I), respectively. Good linearity was obtained for ethambutol and Impurity I in their concentration range both with an <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> value of 0.999. Overall, the method established in this study, utilizing a new detection technique, proved to be accurate and rapid and is applicable for directly detecting Impurity I and ethambutol in quality control.<\/jats:p>","DOI":"10.1002\/bmc.70049","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T21:22:11Z","timestamp":1741728131000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Novel Derivatization\u2010Free Approach for Analysis of (+)2\u2010Aminobutanol and Ethambutol Hydrochloride in Tablets Preparation Using RP\u2010HPLC\u2010NQAD"],"prefix":"10.1002","volume":"39","author":[{"given":"Hao","family":"Ruan","sequence":"first","affiliation":[{"name":"Zhejiang University of Technology  Hangzhou China"},{"name":"Key Laboratory for Core Technology of Generic Drug Evaluation National Medical Product Administration Zhejiang Institute for Food and Drug Control  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1967-9859","authenticated-orcid":false,"given":"Ying","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory for Core Technology of Generic Drug Evaluation National Medical Product Administration Zhejiang Institute for Food and Drug Control  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Yang","sequence":"additional","affiliation":[{"name":"Sanyo fine trading Co, Ltd  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Liu","sequence":"additional","affiliation":[{"name":"Zhejiang Center for Drug and Cosmetics Evaluation  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfeng","family":"Shi","sequence":"additional","affiliation":[{"name":"Key Laboratory for Core Technology of Generic Drug Evaluation National Medical Product Administration Zhejiang Institute for Food and Drug Control  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minglong","family":"Ye","sequence":"additional","affiliation":[{"name":"Key Laboratory for Core Technology of Generic Drug Evaluation National Medical Product Administration Zhejiang Institute for Food and Drug Control  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liya","family":"Hong","sequence":"additional","affiliation":[{"name":"Key Laboratory for Core Technology of Generic Drug Evaluation National Medical Product Administration Zhejiang Institute for Food and Drug Control  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2544-1948","authenticated-orcid":false,"given":"Weike","family":"Su","sequence":"additional","affiliation":[{"name":"Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2013.01.022"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2012.01.036"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/aac.39.3.694"},{"key":"e_1_2_7_5_1","first-page":"2704","volume-title":"European Pharmacopoeia","author":"European Directorate for the Quality of Medicines & HealthCare","year":"2022"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.08.028"},{"issue":"5","key":"e_1_2_7_7_1","first-page":"804","article-title":"HPLC\u2010ELSD Determination of Content and Dissolution of Ethambutol Hydrochloride Tablets","volume":"28","author":"Liu X.","year":"2008","journal-title":"Chinese Journal of Pharmaceutical 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