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However, their physicochemical instability limits broader therapeutic applications. This study aimed to develop an analytical methodology for the simultaneous quantification of these compounds in nanoparticles using liquid chromatography. The suspensions exhibited favorable characteristics, including a particle size of 117\u2009nm, encapsulation efficiency above 60%, zeta potential of 14\u2009mV, and adequate bioactive content. Notably, the system enhanced their photostability under UV radiation. The HPLC\u2010UV method employed a C18 column with a mobile phase of acidic water (pH\u20092.6), methanol, and acetonitrile (64:18:18, v\/v\/v) at 1\u2009mL\/min, with detection at 223\u2009nm, achieving fast and efficient separation. The method was linear, specific, precise, accurate, and robust, in accordance with Brazilian and ICH guidelines. Additionally, the method was stability\u2010indicating, identifying isomeric degradation products, which represent the most common degradation reaction for these compounds. Computational analysis using density functional theory (DFT) further explored the stability of CA and FA. It revealed a strong interaction between the carboxyl group and hydrogen peroxide, suggesting reduced susceptibility to oxidation. These findings provide a foundation for designing stable formulations and enhancing the understanding of antioxidant mechanisms in phenolic compounds.<\/jats:p>","DOI":"10.1002\/bmc.70051","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T06:19:43Z","timestamp":1741760383000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Simultaneous Quantification of Caffeic and Ferulic Acids by HPLC\u2010UV in Nanoparticles: Degradation Profiling and Computational Peroxidation Analysis"],"prefix":"10.1002","volume":"39","author":[{"given":"Igor\u00a0Roesch","family":"Felix","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Centro de Ci\u00eancias da Sa\u00fade Universidade Federal de Santa Maria  Santa Maria RS 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