{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:40:41Z","timestamp":1790880041740,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A rapid and sensitive ultra\u2010high performance liquid chromatography\u2013tandem mass spectrometry (UHPLC\u2010MS\/MS) method for the determination of bisoprolol in human plasma was established and validated. The sample was pretreated by methanol precipitation protein, and the isotope bisoprolol\u2010d<jats:sub>5<\/jats:sub> was used as the internal standard. The chromatographic column was ACQUITY UPLC BEH\u2010C<jats:sub>18<\/jats:sub> column (2.1\u2009\u00d7\u200950\u2009mm, 1.7\u2009\u03bcm), with methanol and 0.2% formic acid aqueous solution as mobile phase for gradient elution. The electrospray ionization (ESI) source was used in the positive ion mode, and the multiple reaction monitoring (MRM) mode was used. The total running time was only 2.00\u2009min. The correlation coefficient was good (<jats:italic>r<\/jats:italic>\u2009&gt;\u20090.99) in the linear range of 0.0200\u201340.0\u2009ng\/mL. The lower limit of quantitation (LLOQ) was 20.0\u2009pg\/mL. The intrabatch and interbatch precisions were not more than 8.9% and 9.2%. The intrabatch and interbatch accuracies were \u22127.9%\u2009~\u20096.3% and \u22126.9%\u2009~\u20095.0%. The method was fully validated including whole blood stability and reinjection reproducibility and successfully applied to the pharmacokinetic study of 5\u2010mg bisoprolol in healthy volunteers, which 93.1% incurred samples reanalysis (ISR) met the criteria. Compared with the reported methods, this method had the highest sensitivity and fast analysis speed.<\/jats:p>","DOI":"10.1002\/bmc.70054","type":"journal-article","created":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T06:19:31Z","timestamp":1743574771000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Rapid and Sensitive Method for the Determination of Bisoprolol in Human Plasma by Ultra Performance Liquid Chromatography\u2013Tandem Mass Spectrometry"],"prefix":"10.1002","volume":"39","author":[{"given":"Yihong","family":"Jiang","sequence":"first","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaling","family":"Niu","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congchao","family":"Ning","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8562-7280","authenticated-orcid":false,"given":"Feng","family":"Qin","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengda","family":"Yan","sequence":"additional","affiliation":[{"name":"The First Hospital of China Medical University  Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumei","family":"Lu","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,2]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"StatPearls","author":"Bazroon A. 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