{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:43:32Z","timestamp":1760060612712,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:00:00Z","timestamp":1744243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY21H280008"],"award-info":[{"award-number":["LY21H280008"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Da\u2010Huang\u2010Zhe\u2010Chong Pills (DHZCPs) have demonstrated efficacy in treating uterine fibroids (UFs), but the mechanisms underlying their action remain unclear. In this study, untargeted serum metabolomic analysis was employed to investigate the therapeutic effects of DHZCP in a rat model of UFs utilizing advanced ultra\u2010performance liquid chromatography tandem quadrupole time\u2010of\u2010flight mass spectrometry (UPLC\u2010Q\u2010TOF\/MS) and pattern recognition. Histopathological examination through H&amp;E staining revealed significant improvements in uterine morphology following DHZCP administration. The levels of four serum hormones, that is, estradiol (E<jats:sub>2<\/jats:sub>), follicle\u2010stimulating hormone (FSH), luteinizing hormone (LH), and progesterone (PROG), tended to shift toward normal in the DHZCP\u2010treated group compared with the model group. Our metabolomic profiling identified 20 distinct endogenous metabolites that were differentially expressed between UF and normal rats, with DHZCP treatment significantly normalizing 16 of these metabolic markers. Comprehensive pathway analysis highlighted three major metabolic pathways affected by DHZCP intervention: tryptophan metabolism, riboflavin metabolism, and arginine\u2010proline metabolism. Notably, spearman correlation analysis indicated that tryptophan, lysoPC (18:1\/0:0), uric acid, and serotonin were strongly positively correlated with serum levels of both E<jats:sub>2<\/jats:sub> and PROG. Additionally, riboflavin, indoleacetic acid, and indole\u20103\u2010propionic acid were positively correlated with E<jats:sub>2<\/jats:sub> levels. Our findings suggest that DHZCP may exert therapeutic effects primarily through the modulation of tryptophan and riboflavin metabolism, providing a solid foundation for its clinical application.<\/jats:p>","DOI":"10.1002\/bmc.70083","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T05:46:31Z","timestamp":1744263991000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Effect and Mechanism of Da\u2010Huang\u2010Zhe\u2010Chong Pills on Uterine Fibroids Using Liquid Chromatography Combined With Mass Spectrometry Metabolomics"],"prefix":"10.1002","volume":"39","author":[{"given":"Yonghua","family":"Hu","sequence":"first","affiliation":[{"name":"Gynaecology Department Yongkang Traditional Chinese Medicine Hospital  Yongkang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xujin","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenmei","family":"Ma","sequence":"additional","affiliation":[{"name":"Gynaecology Department Yongkang Traditional Chinese Medicine Hospital  Yongkang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Gynaecology Department Yongkang Traditional Chinese Medicine Hospital  Yongkang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Yuan","sequence":"additional","affiliation":[{"name":"Gynaecology Department Yongkang Traditional Chinese Medicine Hospital  Yongkang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man","family":"He","sequence":"additional","affiliation":[{"name":"Gynaecology Department Yongkang Traditional Chinese Medicine Hospital  Yongkang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Penghai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Gynaecology Department Yongkang Traditional Chinese Medicine Hospital  Yongkang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6838-0749","authenticated-orcid":false,"given":"Yi","family":"Tao","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,10]]},"reference":[{"issue":"3","key":"e_1_2_11_2_1","first-page":"293","article-title":"Role of Taurine in the Vasculature: An Overview of Experimental and Human Studies","volume":"1","author":"Abebe W.","year":"2011","journal-title":"American Journal of Cardiovascular Disease"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/14728222.2024.2390094"},{"key":"e_1_2_11_4_1","first-page":"108","article-title":"Damage\u2010Associated Molecular Patterns in Patients With Intramural Uterine Fibroids and Infertility","volume":"5","author":"Dubinskaya E. 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