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The separation was achieved on a UPLC HSS T3 column with a mobile phase consisting of methanol and water (with 0.1% formic acid in water), at a flow rate of 0.4\u2009mL\/min. Detection was performed using electrospray ionization (ESI) in positive ion mode, coupled with multiple reaction monitoring (MRM) for quantitative analysis. Rats received oral doses of schaftoside (1\u2009mg\/kg) and isoschaftoside (5\u2009mg\/kg), and the pharmacokinetic profiles of both compounds were compared. The calibration curve for the method demonstrated excellent linearity within the concentration range of 1\u20132000\u2009ng\/mL, with correlation coefficients (<jats:italic>r<\/jats:italic> values) exceeding 0.99. Following intravenous and oral administration, significant differences were observed in the AUC<jats:sub>(0\u2013t)<\/jats:sub> between schaftoside and isoschaftoside, whereas their half\u2010lives (t<jats:sub>1\/2<\/jats:sub>) remained comparable. The absolute bioavailability of schaftoside and isoschaftoside in rat plasma was determined to be 0.95% and 0.22%, respectively.<\/jats:p>","DOI":"10.1002\/bmc.70106","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T18:48:15Z","timestamp":1747075695000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Determination of Schaftoside and Isoschaftoside in Rat Plasma Utilizing UPLC\u2010MS\/MS"],"prefix":"10.1002","volume":"39","author":[{"given":"Jianbo","family":"Li","sequence":"first","affiliation":[{"name":"The Second Affiliated Hospital Zhejiang University School of Medicine Linping Campus  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runrun","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou 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