{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T14:14:31Z","timestamp":1789827271546,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173920"],"award-info":[{"award-number":["82173920"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82003896"],"award-info":[{"award-number":["82003896"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Natural deep eutectic solvents (NADESs) have garnered significant attention for their application in the extraction of natural products. This study proposes a strategy to enhance the extraction efficiency of target compounds from the <jats:italic>Atractylodes macrocephala<\/jats:italic> Koidz\u2013<jats:italic>Astragali Radix<\/jats:italic> (AM\u2010AR) combination, and the crude extracts were employed for subsequent in\u00a0vitro and in\u00a0vivo investigations. The extraction efficiencies of nine NADESs and conventional solvents were compared for isolating calycosin 7\u2010O\u2010glucoside, ononin, quercetin, and atractylenolide III using high\u2010performance liquid chromatography (HPLC) analysis. The extraction parameters were then optimized through response surface methodology (RSM) and genetic algorithm\u2013back propagation neural network (GA\u2010BPNN) models. In addition, the antioxidant activities were evaluated in\u00a0vitro using assays such as 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH), 2,2\u2010azino\u2010bis (3\u2010ethylbenzothiazoline\u20106\u2010sulfonic acid) diammonium salt (ABTS) free radical scavenging, and ferric reducing antioxidant power (FRAP). An in\u00a0vivo rat model of middle cerebral artery occlusion (MCAO) was utilized to assess the biological activities. Among the tested solvents, NADES\u20107 exhibited the highest extraction efficiency, achieving a comprehensive evaluation value of 0.173 and was selected for subsequent experiments. These findings indicate that NADESs can serve as promising alternative solvents for extracting natural products from traditional Chinese medicine (TCM).<\/jats:p>","DOI":"10.1002\/bmc.70122","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T03:07:01Z","timestamp":1747796821000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Optimization of Natural Deep Eutectic Solvents' Extraction Process From <i>Atractylodes macrocephala<\/i>\u2013<i>Astragali Radix<\/i> Using the Dual Mathematical Model and Its Activity Evaluation"],"prefix":"10.1002","volume":"39","author":[{"given":"Wenlong","family":"Huang","sequence":"first","affiliation":[{"name":"School of Life Sciences Zhejiang Chinese Medical University  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingling","family":"Meng","sequence":"additional","affiliation":[{"name":"School of Life 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