{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T23:10:43Z","timestamp":1760051443888,"version":"build-2065373602"},"reference-count":11,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,6,8]],"date-time":"2025-06-08T00:00:00Z","timestamp":1749340800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R50CA211241","U24CA247643"],"award-info":[{"award-number":["R50CA211241","U24CA247643"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ataxia\u2010telangiectasia and Rad3\u2010related (ATR) protein kinase is an essential regulator of the DNA damage response (DDR) at stalled and collapsed replication forks. Tuvusertib (M1774) is a selective, orally available small molecule ATR inhibitor currently in preclinical and clinical development for cancer treatment. This study presents a robust and simple 5\u2010min assay designed for the quantification of single agent tuvusertib in human plasma utilizing liquid chromatography tandem mass spectrometry (LC\u2010MS\/MS). A 20\u2009\u03bcL volume of plasma was subjected to protein precipitation, followed by chromatographic separation using a Phenomenex Synergi Polar\u2010RP (4\u2009\u03bcm, 2.1\u2009\u00d7\u200950\u2009mm) and a gradient mobile phase system consisting of 0.1% formic acid in both water and acetonitrile during a 4\u2010min run time. Mass spectrometric detection was achieved using a SCIEX 6500+ tandem mass spectrometer with electrospray positive\u2010mode ionization. With a stable isotopic internal standard, our assay met the criteria outlined by the Food and Drug Administration guidance for bioanalytical method validation, demonstrating robust performance within the range from 5 to 5000\u2009ng\/mL. This assay will support ongoing and future clinical studies by defining tuvusertib pharmacokinetics.<\/jats:p>","DOI":"10.1002\/bmc.70134","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T11:04:32Z","timestamp":1749726272000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantitation of Tuvusertib (M1774) in Human Plasma by LC\u2010MS\/MS"],"prefix":"10.1002","volume":"39","author":[{"given":"Tien\u00a0V.","family":"Le","sequence":"first","affiliation":[{"name":"Cancer Therapeutics Program UPMC Hillman Cancer Center  Pittsburgh Pennsylvania USA"},{"name":"Department of Pharmaceutical Sciences, School of Pharmacy University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert\u00a0A.","family":"Parise","sequence":"additional","affiliation":[{"name":"Cancer Therapeutics Program UPMC Hillman Cancer Center  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julianne\u00a0L.","family":"Holleran","sequence":"additional","affiliation":[{"name":"Cancer Therapeutics Program UPMC Hillman Cancer Center  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher\u00a0J.","family":"Bakkenist","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Synold","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Therapeutics Research City of Hope Comprehensive Cancer Center  Duarte California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Therapeutics Research City of Hope Comprehensive Cancer Center  Duarte California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory\u00a0M.","family":"Cote","sequence":"additional","affiliation":[{"name":"Mass General Cancer Center  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven\u00a0D.","family":"Gore","sequence":"additional","affiliation":[{"name":"Investigational Drug Branch, Cancer Therapy Evaluation Program, Division of Cancer Treatment and Diagnosis National Cancer Institute  Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8978-9401","authenticated-orcid":false,"given":"Jan\u00a0H.","family":"Beumer","sequence":"additional","affiliation":[{"name":"Cancer Therapeutics Program UPMC Hillman Cancer Center  Pittsburgh Pennsylvania USA"},{"name":"Department of Pharmaceutical Sciences, School of Pharmacy University of Pittsburgh  Pittsburgh Pennsylvania USA"},{"name":"Division of Hematology\u2010Oncology, Department of Medicine University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.09.019"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535\u20107163.MCT\u201023\u20100402"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.02.011"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1081\/jlc\u2010100101455"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000272834"},{"key":"e_1_2_6_7_1","unstructured":"U.S. Department of Health and Human Services Food and Drug Administration.2018. \u201cGuidance for Industry\u2010Bioanalytical Method Validation.\u201d U.S. Department of Health and Human Services; Food and Drug Administration; Center for Drug Evaluation and Research (CDER); Center for Veterinary Medicine (CVM).www.fda.gov\/media\/70858\/download."},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.23761"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201023\u20102409"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.05.031"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1538\u20107445.Am2022\u20102588"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1083430"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70134","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:28:57Z","timestamp":1760048937000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,8]]},"references-count":11,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/bmc.70134"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70134","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2025,6,8]]},"assertion":[{"value":"2025-05-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70134"}}