{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:49:27Z","timestamp":1760060967741,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Understanding the thermodynamic and extra\u2010thermodynamic interactions of a ligand to its target can provide important insights into ligand affinity and efficiency. Using endothelin receptor A (ET<jats:sub>A<\/jats:sub>R) as an example, ET<jats:sub>A<\/jats:sub>R was immobilized on ibrutinib\u2010modified silica gels to prepare affinity stationary phase. After specificity and stability characterization, the affinity column was utilized to investigate the interactions between four ligands with ET<jats:sub>A<\/jats:sub>R. The four ligands were ambrisentan, paeoniflorin (PAE), ferulic acid (FA), and salvianolic acid B (SAB). Nonlinear chromatography was applied to determine the affinities of the ligands with ET<jats:sub>A<\/jats:sub>R at temperature range of 283.15~318.15\u2009K. Entropy change (\u0394<jats:italic>H<\/jats:italic>), enthalpy change (\u0394<jats:italic>S<\/jats:italic>), and Gibbs free energy change (\u0394<jats:italic>G<\/jats:italic>) were determined. The \u0394<jats:italic>H<\/jats:italic> and \u0394<jats:italic>S<\/jats:italic> remain constant for ambrisentan and PAE. While enthalpy\u2013entropy compensation happened for FA and SAB, indicating conformational changes occurred during the binding of them with ET<jats:sub>A<\/jats:sub>R. PAE had similar thermodynamic behaviors to ambrisentan (an approved drug targeting ET<jats:sub>A<\/jats:sub>R for the treatment of pulmonary arterial hypertension), implying that the ligand has greater possibilities to be a lead than FA and SAB. This result was also confirmed by a survey of DrugBank. Together, the method may become an alternative for predicting the druglike property of a newly discovered bioactive compound.<\/jats:p>","DOI":"10.1002\/bmc.70149","type":"journal-article","created":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T00:45:50Z","timestamp":1750293950000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Thermodynamic and Extra\u2010Thermodynamic Investigations of Bioactive Compounds With Endothelin A Receptor by Nonlinear Chromatography"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5467-7509","authenticated-orcid":false,"given":"Chan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pharmacy Administration and Clinical Pharmacy, School of Pharmacy Xi'an Jiaotong University  Shaanxi China"},{"name":"Shaanxi Center for Drug and Vaccine Inspection  Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanbing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shaanxi Pharmaceutical Holding Group Co., Ltd.  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Administration and Clinical Pharmacy, School of Pharmacy Xi'an Jiaotong University  Shaanxi China"},{"name":"Center for Drug Safety and Policy Research Xian Jiaotong University  Xi'an Shaanxi 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