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This research developed a rapid, reliable, and cost\u2010effective LC\u2010MS\/MS method to quantify buspirone, alprazolam, clonazepam, diazepam, and lorazepam in human plasma for therapeutic drug monitoring. Furthermore, compliance with the International Council for Harmonisation (ICH) M10 Bioanalytical Method Validation and Study Sample Analysis ensured the method's reliability. Before injection into the LC\u2010MS\/MS system, the analytes and an internal standard were extracted from plasma through salt\u2010assisted liquid\u2013liquid microextraction (SALLME). The lower limit of quantification (LLOQ) was determined as 0.5, 2.5, 2, 30, and 10\u2009ng\/mL for buspirone, alprazolam, clonazepam, diazepam, and lorazepam, respectively. The calibration curve ranges are 0.5\u201350\u2009ng\/mL for buspirone, 2.5\u2013250\u2009ng\/mL for alprazolam, 2\u2013200\u2009ng\/mL for clonazepam, 30\u20133000\u2009ng\/mL for diazepam, and 10\u20131000\u2009ng\/mL for lorazepam, respectively, with correlation coefficients &gt;\u20090.99. Its suitability for therapeutic drug monitoring was demonstrated using the method to determine drug concentration levels in real patients. The developed method has been observed to achieve a high analytical greenness metric approach and software (AGREE) score for the greenness profile.<\/jats:p>","DOI":"10.1002\/bmc.70155","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T22:30:43Z","timestamp":1750804243000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Simultaneous Determination of Buspirone, Alprazolam, Clonazepam, Diazepam, and Lorazepam by LC\u2010MS\/MS: Evaluation of Greenness Using AGREE Tool"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1195-0939","authenticated-orcid":false,"given":"Aykut","family":"Kul","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy Istanbul University  Istanbul 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