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UPLC\u2010Orbitrap\u2010Exploris MS characterized 156 bioactive components in QZT. Subsequently, an NAFLD model was established in ICR mice via 8\u2010week feeding with a high\u2010glucose and high\u2010fat diet. The NAFLD mice were randomly divided into four groups. After 8\u2009weeks of drug administration, compared with the control group, the model group showed significant NAFLD\u2010related liver pathological changes, manifested as elevated blood lipid parameters (TC, TG, and LDL) and decreased HDL. Liver injury markers (ALT and AST) and serum inflammatory cytokines (IL\u20101\u03b2, IL\u20106, and TNF\u2010\u03b1) were significantly increased. Molecular analysis indicated that the expression of p\u2010IRs\u20101, PI3K, and p\u2010Akt was decreased in liver tissue, while the expression of SREBP, NLRP3, caspase1, and GSDMD was increased. QZT treatment alleviated these changes, significantly improving NAFLD pathology, normalizing lipid profiles, and regulating the expression of these molecular markers (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). QZT improved insulin resistance, regulated lipid metabolism, and reduced inflammatory response, and its mechanism might be associated with the regulation of IRs\u20101 and NLRP3 pathways.<\/jats:p>","DOI":"10.1002\/bmc.70156","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T05:35:13Z","timestamp":1753335313000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Insights Into the Mechanism of Action for Qingzhi Decoction for the Treatment of Non\u2010Alcoholic Fatty Liver Disease via Regulation of the IRS\/PI3K\/Akt Signaling Pathway and the NRLP3 Inflammasome"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3201-405X","authenticated-orcid":false,"given":"Jiejun","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Pharmacy Zhongda Hospital Southeast University  Nanjing 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