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However, the complexity of the chemical composition of traditional Chinese medicine poses challenges to controlling the quality of ECP. This study aimed to predict and validate the quality markers (Q\u2010markers) of ECP. First, we performed high\u2010performance liquid chromatography (HPLC) to establish fingerprints of 26 batches of ECP and identify common peaks. Subsequently, quality\u2010differentiating components were identified by chemometrics, and potential Q\u2010markers of ECP were further validated through network pharmacology and molecular docking. The five candidate Q\u2010markers\u2014hesperidin, liquiritin apioside, nobiletin, liquiritin, and tangeretin\u2014were selected via multivariate statistical analysis. The results of network pharmacology analysis and molecular docking revealed that the five candidate Q\u2010markers acted on multiple targets and pathways, with high binding ability with core targets. Notably, in\u00a0vitro experiments validated the anti\u2010tumor effects of liquiritin apioside, which suppressed proliferation and invasion of human prostate cancer PC\u20103 cells and induced their apoptosis. In this study, we predicted and validated the Q\u2010markers of ECP, providing a scientific basis for the comprehensive evaluation of the quality of ECP.<\/jats:p>","DOI":"10.1002\/bmc.70163","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T06:18:54Z","timestamp":1753165134000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Predictive Analysis and Activity Validation of Quality Markers in Erchen Pill"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0083-0966","authenticated-orcid":false,"given":"Yingxin","family":"Liu","sequence":"first","affiliation":[{"name":"Hunan Traditional Chinese Medical College  Zhuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xintian","family":"Lu","sequence":"additional","affiliation":[{"name":"Hunan Traditional Chinese Medical College  Zhuzhou 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