{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T06:10:19Z","timestamp":1773382219158,"version":"3.50.1"},"reference-count":216,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002385","name":"Ministry of Higher Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002385","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004726","name":"Ministry of Health, British Columbia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004726","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Human brain tumors were commonly monitored in hospital\/clinical laboratories by immunohistochemistry (IHC) technique, which provides major insights into their classification. However, this technique remains laborious and still shows pitfalls. Therefore, the current study was endeavored to reveal the assets of the application of high\u2010throughput mass spectrometry (MS) for medical diagnosis. In this study, we focused on the Grade IV astrocytoma and meningioma brain tumors. The collected specimens were first monitored for histopathological diagnosis, followed by IHC staining for the characterization of stemness gene marker, then analyzed by a shotgun proteomic\u2013based approach with high\u2010resolution tandem MS. The IHC analysis only confirmed the histopathological diagnosis, whereas the proteomic analysis unraveled several differently expressed proteins. By bioinformatics, the major enriched pathways and the significance of each protein with its meaningful relationships were identified. The key hub genes were allied for prognostic biomarkers of malignant, metastatic, and invasive forms of cancer with poor prognosis. Overall, the high\u2010throughput MS technique is the most powerful tool to achieve medical analysis at high sensitivity and accuracy and in a very straightforward and timely manner. Hence, its medical implementation in the hospital management system is imperative to counteract the caveats of traditional diagnostic methods and improve the quality of healthcare performance and therapeutic targets.<\/jats:p>","DOI":"10.1002\/bmc.70170","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T09:47:50Z","timestamp":1754473670000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Application of the Mass Spectrometry\u2013High\u2010Throughput Technique Over the Immunohistochemical Analysis for Human Brain Tumor Diagnosis and Prognosis: Insights Into Biomarkers' Identification for the Case Study of Grade IV Astrocytomas and Meningiomas"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5085-4766","authenticated-orcid":false,"given":"Kaouthar","family":"Louati","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Laboratory of Chemical, Galenic and Pharmacological Drug Development University of Monastir  Monastir Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatma","family":"Kolsi","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Habib Bourguiba University Hospital  Sfax Tunisia"},{"name":"Faculty of Medicine University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manel","family":"Mellouli","sequence":"additional","affiliation":[{"name":"Laboratory of Pathological Anatomy and Cytology Habib Bourguiba University Hospital  Sfax Tunisia"},{"name":"Faculty of Medicine University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanen","family":"Louati","sequence":"additional","affiliation":[{"name":"College of Administrative and Financial Sciences Saudi Electronic University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rim","family":"Kallel","sequence":"additional","affiliation":[{"name":"Laboratory of Pathological Anatomy and Cytology Habib Bourguiba University Hospital  Sfax Tunisia"},{"name":"Faculty of Medicine University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0307-0261","authenticated-orcid":false,"given":"Rania","family":"Zribi","sequence":"additional","affiliation":[{"name":"Faculty of Letters and Humanities University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahdi","family":"Borni","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Habib Bourguiba University Hospital  Sfax Tunisia"},{"name":"Faculty of Medicine University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leila\u00a0Sellami","family":"Hakim","sequence":"additional","affiliation":[{"name":"Laboratory of Pathological Anatomy and Cytology Habib Bourguiba University Hospital  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amina","family":"Maalej","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Bioprocesses Centre of Biotechnology of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirine","family":"Choura","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Bioprocesses Centre of Biotechnology of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Chamkha","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Bioprocesses Centre of Biotechnology of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sami","family":"Sayadi","sequence":"additional","affiliation":[{"name":"Biotechnology Program, Center for Sustainable Development, College of Arts and Sciences Qatar University  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zouheir","family":"Khemakhem","sequence":"additional","affiliation":[{"name":"Legal Medicine Department Habib Bourguiba University Hospital  Sfax Tunisia"},{"name":"Faculty of Medicine University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahya\u00a0Sellami","family":"Boudawara","sequence":"additional","affiliation":[{"name":"Laboratory of Pathological Anatomy and Cytology Habib Bourguiba University Hospital  Sfax Tunisia"},{"name":"Faculty of Medicine University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed\u00a0Zaher","family":"Boudawara","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Habib Bourguiba University Hospital  Sfax Tunisia"},{"name":"Faculty of Medicine University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaouthar","family":"Zribi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Laboratory of Chemical, Galenic and Pharmacological Drug Development University of Monastir  Monastir Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fathi","family":"Safta","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Laboratory of Chemical, Galenic and Pharmacological Drug Development University of Monastir  Monastir Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,6]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13100801"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12061153"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.20892\/j.issn.2095\u20103941.2017.0024"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201042682\u20109"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935\u2010018\u20100571\u20106"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.5137\/1019\u20105149.jtn.9852\u201013.0"},{"issue":"2","key":"e_1_2_13_8_1","first-page":"585","article-title":"Adhesion Cell Molecules as Potential Markers of Aggressiveness in Meningiomas","volume":"55","author":"Arsene D.","year":"2014","journal-title":"Romanian Journal of Morphology and Embryology"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14215435"},{"issue":"10","key":"e_1_2_13_10_1","first-page":"7206","article-title":"Immunohistochemical Characterization of Brain\u2010Invasive Meningiomas","volume":"7","author":"Backer\u2010Gr\u00f8ndahl T.","year":"2014","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555\u2010024\u201010216\u20104"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.70655"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2024.101609"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033\u2010022\u201007963\u20104"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200307098"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.188045"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/00498254.2012.662726"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9010010"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp101"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt019"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12014\u2010023\u201009424\u2010x"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009238"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/nlx008"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/2767\u20109764.crc\u201022\u20100337"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10014\u2010014\u20100199\u20108"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2017.03.002"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.2217\/fon\u20102018\u20100006"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1227\/01.neu.0000170440.32720.77"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10147\u2010017\u20101103\u20104"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102990.2000.00223.x"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15020555"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.202100008"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010023\u201001615\u20109"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17458"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21807"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2014.08.015"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/14789450.2017.1365604"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2016.06.005"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11101928"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20102576"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2021.03.009"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00045.2020"},{"issue":"5","key":"e_1_2_13_43_1","first-page":"5485","article-title":"Overexpression of HLA\u2010DR Is Associated With Prognosis of Glioma Patients","volume":"8","author":"Diao J.","year":"2015","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay0939"},{"key":"e_1_2_13_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40487\u2010022\u201000205\u20104"},{"key":"e_1_2_13_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2013.00241"},{"key":"e_1_2_13_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10014\u2010015\u20100212\u2010x"},{"key":"e_1_2_13_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41016\u2010017\u20100090\u20107"},{"key":"e_1_2_13_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e38182"},{"key":"e_1_2_13_50_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.can\u201013\u20101942"},{"key":"e_1_2_13_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.1997.tb01193.x"},{"key":"e_1_2_13_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035\u2010017\u20100800\u20103"},{"issue":"7","key":"e_1_2_13_53_1","first-page":"2585","article-title":"Temozolomide and Treatment of Malignant Glioma","volume":"6","author":"Friedman H. S.","year":"2000","journal-title":"Clinical Cancer Research"},{"key":"e_1_2_13_54_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath\u20102019\u2010206269"},{"key":"e_1_2_13_55_1","doi-asserted-by":"publisher","DOI":"10.5137\/1019\u20105149.jtn.10800\u201014.2"},{"key":"e_1_2_13_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25158100"},{"key":"e_1_2_13_57_1","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.2014.939635"},{"key":"e_1_2_13_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873\u20103468.14191"},{"key":"e_1_2_13_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25052743"},{"key":"e_1_2_13_60_1","doi-asserted-by":"publisher","DOI":"10.2174\/1385272817888131118162725"},{"key":"e_1_2_13_61_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.can\u201003\u20103424"},{"key":"e_1_2_13_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010022\u201004185\u20106"},{"key":"e_1_2_13_63_1","doi-asserted-by":"publisher","DOI":"10.21037\/cco.2017.06.11"},{"key":"e_1_2_13_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416\u2010020\u20100814\u2010x"},{"key":"e_1_2_13_65_1","doi-asserted-by":"publisher","DOI":"10.1042\/bsr20171456"},{"key":"e_1_2_13_66_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935\u2010021\u201002030\u20107"},{"key":"e_1_2_13_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201056493\u20105"},{"key":"e_1_2_13_68_1","doi-asserted-by":"publisher","DOI":"10.1155\/2001\/327047"},{"key":"e_1_2_13_69_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.737523"},{"key":"e_1_2_13_70_1","doi-asserted-by":"publisher","DOI":"10.1080\/13102818.2022.2053342"},{"key":"e_1_2_13_71_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1203359"},{"key":"e_1_2_13_72_1","doi-asserted-by":"publisher","DOI":"10.4103\/egjp.egjp_44_21"},{"key":"e_1_2_13_73_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15194898"},{"key":"e_1_2_13_74_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4578373"},{"key":"e_1_2_13_75_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.561287"},{"key":"e_1_2_13_76_1","doi-asserted-by":"publisher","DOI":"10.14336\/ad.2019.0519"},{"key":"e_1_2_13_77_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods10081708"},{"key":"e_1_2_13_78_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.828373"},{"key":"e_1_2_13_79_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.648384"},{"key":"e_1_2_13_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.12.013"},{"key":"e_1_2_13_81_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2017.4191"},{"key":"e_1_2_13_82_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/not028"},{"key":"e_1_2_13_83_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.456"},{"key":"e_1_2_13_84_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401\u2010018\u20101844\u20109"},{"key":"e_1_2_13_85_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.898083"},{"key":"e_1_2_13_86_1","doi-asserted-by":"publisher","DOI":"10.14791\/btrt.2014.2.1.7"},{"key":"e_1_2_13_87_1","doi-asserted-by":"publisher","DOI":"10.5414\/np301150"},{"key":"e_1_2_13_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.inat.2020.100901"},{"key":"e_1_2_13_89_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.747333"},{"key":"e_1_2_13_90_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noae150"},{"key":"e_1_2_13_91_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060\u2010017\u20102376\u20101"},{"key":"e_1_2_13_92_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020626"},{"key":"e_1_2_13_93_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202301714"},{"key":"e_1_2_13_94_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201083553\u20107"},{"key":"e_1_2_13_95_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.20894"},{"key":"e_1_2_13_96_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010021\u201003547\u20105"},{"key":"e_1_2_13_97_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935\u2010024\u201003618\u20105"},{"key":"e_1_2_13_98_1","doi-asserted-by":"publisher","DOI":"10.36922\/cp.4987"},{"key":"e_1_2_13_99_1","doi-asserted-by":"publisher","DOI":"10.14791\/btrt.2013.1.2.85"},{"key":"e_1_2_13_100_1","doi-asserted-by":"publisher","DOI":"10.1016\/b978\u20100\u201012\u2010391940\u20103.00001\u20109"},{"key":"e_1_2_13_101_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800162"},{"key":"e_1_2_13_102_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010\u2010013\u20101699\u20102"},{"key":"e_1_2_13_103_1","doi-asserted-by":"publisher","DOI":"10.1515\/med\u20102022\u20100572"},{"key":"e_1_2_13_104_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2023.108458"},{"key":"e_1_2_13_105_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202203423"},{"key":"e_1_2_13_106_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1119945"},{"key":"e_1_2_13_107_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2024.09.002"},{"key":"e_1_2_13_108_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.3c00395"},{"key":"e_1_2_13_109_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.4c00804"},{"key":"e_1_2_13_110_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noab106"},{"key":"e_1_2_13_111_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986709787846569"},{"key":"e_1_2_13_112_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.636614"},{"key":"e_1_2_13_113_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201000366\u20108"},{"issue":"11","key":"e_1_2_13_114_1","first-page":"6702","article-title":"The Oncogenic Potential of Small Nuclear Ribonucleoprotein Polypeptide G: A Comprehensive and Perspective View","volume":"11","author":"Mabonga L.","year":"2019","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_13_115_1","doi-asserted-by":"publisher","DOI":"10.4103\/2277\u20108632.154262"},{"key":"e_1_2_13_116_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5347"},{"key":"e_1_2_13_117_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2019.00181"},{"key":"e_1_2_13_118_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.03.061"},{"key":"e_1_2_13_119_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.ccr\u201004\u20102550"},{"key":"e_1_2_13_120_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0002\u20109440(10)64342\u20108"},{"key":"e_1_2_13_121_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2024.05.001"},{"key":"e_1_2_13_122_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.1c00099"},{"key":"e_1_2_13_123_1","volume-title":"A Gene Annotation & Analysis Resource","author":"METASCAPE","year":"2019"},{"key":"e_1_2_13_124_1","doi-asserted-by":"publisher","DOI":"10.4103\/ajns.ajns_185_19"},{"key":"e_1_2_13_125_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2019.01.006"},{"key":"e_1_2_13_126_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jocn.2016.05.012"},{"key":"e_1_2_13_127_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.701933"},{"key":"e_1_2_13_128_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010025\u201002323\u20100"},{"key":"e_1_2_13_129_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/532451"},{"key":"e_1_2_13_130_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/192362"},{"key":"e_1_2_13_131_1","volume-title":"PubChem Pathway Summary for Pathway R\u2010HSA\u20109716542, Signaling by Rho GTPases, Miro GTPases and RHOBTB3, Source: Reactome","author":"National Center for Biotechnology Information","year":"2025"},{"key":"e_1_2_13_132_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586\u2010024\u201000318\u20102"},{"key":"e_1_2_13_133_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wneu.2018.09.177"},{"key":"e_1_2_13_134_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12691"},{"key":"e_1_2_13_135_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.03.007"},{"key":"e_1_2_13_136_1","doi-asserted-by":"publisher","DOI":"10.1038\/83358"},{"key":"e_1_2_13_137_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15707"},{"key":"e_1_2_13_138_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11040466"},{"key":"e_1_2_13_139_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2017.06.015"},{"key":"e_1_2_13_140_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13402\u2010019\u201000447\u2010x"},{"key":"e_1_2_13_141_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10092290"},{"issue":"3","key":"e_1_2_13_142_1","first-page":"131","article-title":"The Role of Wnt Signalling in Angiogenesis","volume":"38","author":"Olsen J. J.","year":"2017","journal-title":"Clinical Biochemist Reviews"},{"key":"e_1_2_13_143_1","volume-title":"PANTHER database (Version 19.0)","year":"2025"},{"key":"e_1_2_13_144_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2021.188667"},{"key":"e_1_2_13_145_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1044\u2010579x(02)00023\u20108"},{"key":"e_1_2_13_146_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2021.08.013"},{"key":"e_1_2_13_147_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph18010102"},{"key":"e_1_2_13_148_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.m111.013565"},{"issue":"3","key":"e_1_2_13_149_1","first-page":"130","article-title":"The Study of \u201cDihydropyrimidinase Related Proteins (DRPs)\u201d expression Changes Influence in Malignant Astrocytoma Brain Tumor","volume":"7","author":"Pooladi M.","year":"2014","journal-title":"Iranian Journal of Cancer Prevention"},{"key":"e_1_2_13_150_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.tir119.001720"},{"key":"e_1_2_13_151_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof9080790"},{"key":"e_1_2_13_152_1","doi-asserted-by":"publisher","DOI":"10.1159\/000527382"},{"key":"e_1_2_13_153_1","volume-title":"Recent Advances in Analytical Chemistry","author":"Rochat B.","year":"2018"},{"key":"e_1_2_13_154_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418\u2010017\u20101144\u2010z"},{"key":"e_1_2_13_155_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2022.153821"},{"key":"e_1_2_13_156_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010024\u201012750\u20104"},{"key":"e_1_2_13_157_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19041045"},{"key":"e_1_2_13_158_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41417\u2010022\u201000571\u20101"},{"key":"e_1_2_13_159_1","doi-asserted-by":"publisher","DOI":"10.1080\/14789450.2017.1345632"},{"key":"e_1_2_13_160_1","doi-asserted-by":"publisher","DOI":"10.1007\/s44250\u2010024\u201000163\u2010w"},{"key":"e_1_2_13_161_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25189880"},{"key":"e_1_2_13_162_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.03.064"},{"key":"e_1_2_13_163_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes16050600"},{"key":"e_1_2_13_164_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2017.00043"},{"key":"e_1_2_13_165_1","volume-title":"STRING database (Version 12.0)","year":"2023"},{"key":"e_1_2_13_166_1","doi-asserted-by":"publisher","DOI":"10.1200\/jco.2007.11.8554"},{"key":"e_1_2_13_167_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780428"},{"key":"e_1_2_13_168_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060\u2010011\u20100729\u20108"},{"key":"e_1_2_13_169_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2025.1531465"},{"key":"e_1_2_13_170_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2021.101051"},{"key":"e_1_2_13_171_1","doi-asserted-by":"publisher","DOI":"10.1177\/11795549211038256"},{"key":"e_1_2_13_172_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.844866"},{"key":"e_1_2_13_173_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458511399614"},{"key":"e_1_2_13_174_1","volume-title":"The Human Protein Atlas (version 24.0)","year":"2024"},{"key":"e_1_2_13_175_1","volume-title":"\u201cQ Exactive HF\u2010X Hybrid Quadrupole\u2010Orbitrap\u2010MS System\u201d, PRODUCT SPECIFICATION Data\u2010Sheet","author":"The Thermo Scientific","year":"2017"},{"key":"e_1_2_13_176_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2019.12.001"},{"key":"e_1_2_13_177_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586\u2010022\u201000175\u2010x"},{"key":"e_1_2_13_178_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41417\u2010023\u201000680\u20105"},{"key":"e_1_2_13_179_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00701\u2010022\u201005301\u2010y"},{"key":"e_1_2_13_180_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bspc.2022.103922"},{"key":"e_1_2_13_181_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073\u2010024\u201001315\u20106"},{"key":"e_1_2_13_182_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2017.01.009"},{"key":"e_1_2_13_183_1","doi-asserted-by":"publisher","DOI":"10.12703\/r\/10\u201045"},{"key":"e_1_2_13_184_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.01.040"},{"key":"e_1_2_13_185_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12040892"},{"key":"e_1_2_13_186_1","doi-asserted-by":"publisher","DOI":"10.46883\/onc.2021.3507.0402"},{"key":"e_1_2_13_187_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.73365"},{"key":"e_1_2_13_188_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.962481"},{"key":"e_1_2_13_189_1","doi-asserted-by":"publisher","DOI":"10.4103\/glioma.glioma_11_22"},{"key":"e_1_2_13_190_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e24464"},{"key":"e_1_2_13_191_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12014\u2010024\u201009452\u20101"},{"key":"e_1_2_13_192_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010030\u201035723\u20108_1"},{"key":"e_1_2_13_193_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.994440"},{"key":"e_1_2_13_194_1","doi-asserted-by":"publisher","DOI":"10.1080\/19491034.2024.2423660"},{"key":"e_1_2_13_195_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina61010096"},{"key":"e_1_2_13_196_1","doi-asserted-by":"publisher","DOI":"10.5137\/1019\u20105149.JTN.21234\u201017.2"},{"key":"e_1_2_13_197_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12920\u2010023\u201001466\u20102"},{"key":"e_1_2_13_198_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565\u2010024\u201009866\u20105"},{"key":"e_1_2_13_199_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41435\u2010025\u201000327\u20105"},{"key":"e_1_2_13_200_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.711965"},{"key":"e_1_2_13_201_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.01.170"},{"key":"e_1_2_13_202_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bj.2022.03.013"},{"key":"e_1_2_13_203_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/1498924"},{"key":"e_1_2_13_204_1","doi-asserted-by":"publisher","DOI":"10.1134\/s1061934821140094"},{"key":"e_1_2_13_205_1","doi-asserted-by":"publisher","DOI":"10.3390\/cimb46040214"},{"key":"e_1_2_13_206_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20100716\u20103541\u20104_1"},{"key":"e_1_2_13_207_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.09.038"},{"key":"e_1_2_13_208_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr3003533"},{"key":"e_1_2_13_209_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231911989"},{"key":"e_1_2_13_210_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12382"},{"key":"e_1_2_13_211_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109369"},{"key":"e_1_2_13_212_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2023.11892"},{"key":"e_1_2_13_213_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201009234\u20106"},{"key":"e_1_2_13_214_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.87272"},{"key":"e_1_2_13_215_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2024.1365655"},{"key":"e_1_2_13_216_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2025.1586137"},{"key":"e_1_2_13_217_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2021.103283"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70170","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:26:00Z","timestamp":1760048760000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,6]]},"references-count":216,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/bmc.70170"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70170","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,6]]},"assertion":[{"value":"2025-03-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70170"}}