{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T00:15:27Z","timestamp":1760746527472,"version":"build-2065373602"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In the booming era of nanomedicines, Caco\u20102 intestinal permeability experimental models allow characterizing intestinal permeabilities of nanopharmaceuticals and are conducted in the presence of cell culture media (CCM) and stabilizers. Methods for simultaneous quantification of furosemide (FRS) and nateglinide (NTG) in Caco\u20102 experiments are lacking. We developed and validated a novel, simple, and practical reversed\u2010phase high\u2010performance liquid chromatography (RP\u2010HPLC) method with UV detection to simultaneously quantify FRS and NTG in the presence of CCM and stabilizers (Povidone K\u201025, Pluronic F\u201038) for Caco\u20102 intestinal permeability experiments exploring nanoformulations. FRS and NTG nanosuspensions were prepared using high\u2010speed ultrasonic homogenization top\u2013down technique. Caco\u20102 cell culture studies were conducted. ICH Q2(R1) guideline requirements (i.e., selectivity, specificity, accuracy, precision, robustness, stability) were met. System suitability and linearity (FRS: 5.12\u201340.00\u2009\u03bcg\/mL; NTG: 7.31\u201350.00\u2009\u03bcg\/mL), with high determination coefficients (&gt;0.999), and short retention times (FRS: 3.3\u2009min; NTG: 5.3\u2009min) were achieved. NTG and FRS in Caco\u20102 samples were successfully simultaneously quantified, proving usefulness for nanodrug research applications. Varying stabilizers impact intestinal permeability. This work simulates pragmatic conditions, as cardiovascular patients are co\u2010administered these drugs, paving the pathway to using our novel method for a wide range of \u201cbench\u2010to\u2010bedside\u201d nanomedicine research designs to tackle the challenges of polypharmacy in this complex population.<\/jats:p>","DOI":"10.1002\/bmc.70229","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T21:17:14Z","timestamp":1759958234000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Isocratic RP\u2010HPLC Method for Simultaneous Quantification of Nateglinide and Furosemide: Application to In\u00a0Vitro Caco\u20102 Intestinal Permeability Studies of Nanodrugs"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4305-9614","authenticated-orcid":false,"given":"Fatima\u00a0Khalifa","family":"Al\u2010Sulaiti","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Pharmaceutical Technology Department Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5828-4697","authenticated-orcid":false,"given":"Aysegul","family":"Dogan","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Department of Analytical Chemistry Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8952-641X","authenticated-orcid":false,"given":"Fatma","family":"Kir","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Pharmaceutical Technology Department Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5736-5906","authenticated-orcid":false,"given":"Selma","family":"Sahin","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Pharmaceutical Technology Department Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104347(98)00058\u20109"},{"key":"e_1_2_9_3_1","article-title":"Formulation and Optimization of Repaglinide Nanoparticles Using Microfluidics for Enhanced Bioavailability and Management of Diabetes","volume":"11","author":"Ahmad M.","year":"2023","journal-title":"Biomedicine"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.37285\/ijpsn.2014.7.4.11"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2017.01.005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0731\u20107085(02)00680\u20105"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-20665-8_28"},{"key":"e_1_2_9_8_1","first-page":"267","article-title":"Development and Validation of RP\u2010HPLC Method for Metformin Hydrochloride and Nateglinide in Bulk and Combined Dosage Form","volume":"8","author":"Chengalva P.","year":"2016","journal-title":"International Journal of Pharmacy and Pharmaceutical Sciences"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.5599\/admet.881"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40200\u2010024\u201001475\u20107"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmz116"},{"key":"e_1_2_9_12_1","first-page":"38","article-title":"Getting the Peaks Perfect: System Suitability for HPLC","volume":"10","author":"Dong M.","year":"2001","journal-title":"Today's Chemist at Work"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2025.124449"},{"volume-title":"European Pharmacopoeia","year":"2007","author":"European Pharmacopoeia Commission","key":"e_1_2_9_14_1"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-008055232-3.62248-2"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2018.03.005"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp\u20102022\u20100265"},{"key":"e_1_2_9_18_1","unstructured":"Huey N. 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