{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T09:40:02Z","timestamp":1760694002920,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T00:00:00Z","timestamp":1759536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003488","name":"Henan Polytechnic University","doi-asserted-by":"publisher","award":["B2018\u201044"],"award-info":[{"award-number":["B2018\u201044"]}],"id":[{"id":"10.13039\/501100003488","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011447","name":"Science and Technology Department of Henan Province","doi-asserted-by":"publisher","award":["252102310455"],"award-info":[{"award-number":["252102310455"]}],"id":[{"id":"10.13039\/501100011447","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82304314"],"award-info":[{"award-number":["82304314"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["252300421382"],"award-info":[{"award-number":["252300421382"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Dioscorea opposita<\/jats:italic> Thunb. cv. Tiegun (DTT) is used daily as food and medicine. However, there is confusion in removing the red flesh of DTT (RF), as the nutrient phytochemicals of RF are unclear. This study aimed to reveal the phytochemicals and antioxidant capabilities of RF. An ultra\u2010high\u2010performance liquid chromatography\u2010quadrupole Orbitrap high\u2010resolution mass spectrometry (UHPLC\u2010Q\u2010Orbitrap HRMS) approach was employed to determine the comprehensive phytochemicals of RF, and the antioxidant activities (DPPH, ABTS, and FRAP) of the extracts were evaluated. A total of 249 phytochemicals were detected, including 150 primary metabolites, 68 secondary metabolites, and 31 other compounds. Notably, 166 differential metabolites (163 upregulated) were identified in RF relative to WF (white flesh). RF significantly increased the contents of primary metabolites (such as lipids, organic acids, and amino acids) and secondary metabolites (such as flavonoids and phenolic acids). RF showed higher antioxidant activity (DPPH, ABTS, and FRAP) than WF. The correlation analysis identified that most of the differential metabolites were significantly positively correlated with the antioxidant capacity. This is the first systematic and comprehensive qualitative analysis of the phytochemicals of RF. This study expands our knowledge of the bioactive constituents of RF and highlights the potential nutritional and medicinal values of DTT.<\/jats:p>","DOI":"10.1002\/bmc.70232","type":"journal-article","created":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T13:34:21Z","timestamp":1759584861000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive Analysis of Bioactive Phytoconstituents in Red Flesh of <i>Dioscorea opposita<\/i> Thunb. by UHPLC\u2010Q\u2010Orbitrap\u2010HRMS"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6828-5619","authenticated-orcid":false,"given":"Lanping","family":"Yang","sequence":"first","affiliation":[{"name":"School of Medicine Henan Polytechnic University  Jiaozuo P. R. China"},{"name":"Jiaozuo Key Laboratory for Huaiyao Comprehensive Development Henan Polytechnic University  Jiaozuo P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine Henan Polytechnic University  Jiaozuo P. R. China"},{"name":"Jiaozuo Key Laboratory for Huaiyao Comprehensive Development Henan Polytechnic University  Jiaozuo P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenzhen","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Medicine Henan Polytechnic University  Jiaozuo P. R. China"},{"name":"Jiaozuo Key Laboratory for Huaiyao Comprehensive Development Henan Polytechnic University  Jiaozuo P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangyang","family":"Zhai","sequence":"additional","affiliation":[{"name":"School of Medicine Henan Polytechnic University  Jiaozuo P. R. China"},{"name":"Jiaozuo Key Laboratory for Huaiyao Comprehensive Development Henan Polytechnic University  Jiaozuo P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenhui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine Henan Polytechnic University  Jiaozuo P. R. China"},{"name":"Jiaozuo Key Laboratory for Huaiyao Comprehensive Development Henan Polytechnic University  Jiaozuo P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"School of Medicine Henan Polytechnic University  Jiaozuo P. R. China"},{"name":"Jiaozuo Key Laboratory for Huaiyao Comprehensive Development Henan Polytechnic University  Jiaozuo P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenzhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medicine Henan Polytechnic University  Jiaozuo P. R. China"},{"name":"Jiaozuo Key Laboratory for Huaiyao Comprehensive Development Henan Polytechnic University  Jiaozuo P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4639-8723","authenticated-orcid":false,"given":"Baohua","family":"Hou","sequence":"additional","affiliation":[{"name":"School of Medicine Henan Polytechnic University  Jiaozuo P. R. China"},{"name":"Jiaozuo Key Laboratory for Huaiyao Comprehensive Development Henan Polytechnic University  Jiaozuo P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baobao","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medicine Henan Polytechnic University  Jiaozuo P. R. China"},{"name":"Jiaozuo Key Laboratory for Huaiyao Comprehensive Development Henan Polytechnic University  Jiaozuo P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingchun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Beijing Bencaoyuan Pharmaceutical Co. Ltd  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dehong","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangdong Lecaoyuan Health Industry Co. Ltd  Guangzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miaoqing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangdong Lecaoyuan Health Industry Co. Ltd  Guangzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangdong Lecaoyuan Health Industry Co. Ltd  Guangzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2024.101159"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10071129"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/lam.13420"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106882\u201014\u201035"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10653\u2010019\u201000437\u2010w"},{"key":"e_1_2_9_7_1","first-page":"1597","article-title":"Comparison of the Quality of Dioscorea opposita Thunb. Cv. Tiegun Between Authentic and Non Authentic Areas","volume":"59","author":"Han X.","year":"2018","journal-title":"Journal of Zhejiang Agricultural Sciences"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2024.116502"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.140534"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c00267"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/2211\u20105463.13295"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.70023"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5376"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1582"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7076"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201800780"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.595516"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.19345\/j.cnki.1674\u20107909.2013.12.056"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5726"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3212"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/d3ay02251j"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203649"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.70036"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.132873"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.70086"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.70085"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.202300001"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2022.113321"},{"key":"e_1_2_9_29_1","first-page":"481","article-title":"Effect of Nano Yam Polysaccharide on the Blood Glucose and Blood Lipid in Rats","volume":"33","author":"Yu L.","year":"2020","journal-title":"Pakistan Journal of Pharmaceutical Sciences"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.134111"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2ay01453j"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.08.035"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1200071"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T08:58:26Z","timestamp":1760691506000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,4]]},"references-count":32,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/bmc.70232"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70232","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2025,10,4]]},"assertion":[{"value":"2025-04-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70232"}}