{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T10:47:56Z","timestamp":1774954076830,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to develop a high\u2010throughput and high\u2010sensitivity method for simultaneously determining trimethoprim (TMP), sulfamethoxazole (SMX), and its metabolite acetyl\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010sulfamethoxazole (NSMX) in critically ill patients' plasma. The samples were precipitated using methanol and then purified using the Oasis PRiME HLB solid\u2010phase extraction column. The mobile phase consisted of a mixture of 40% 0.1% formic acid aqueous solution and 60% acetonitrile, with a flow rate of 0.35\u2009mL\/min. Analyses were conducted using UPLC\u2010Q\u2010Exactive Plus Orbitrap mass spectrometry in positive ion mode. With only 50\u2009\u03bcL of plasma required for accurate quantification, this method was applicable for therapeutic drug monitoring (TDM). Validation demonstrated precision and accuracy within 15% of nominal values. The samples remained stable for 12\u2009h at 4\u00b0C and \u221220\u00b0C and for 15\u2009days at \u221220\u00b0C and \u221280\u00b0C, which ensured the reliability of the method during sample storage and handling under different environmental conditions. The method can be applied for concentration monitoring and pharmacokinetic studies, enabling TDM and dose adjustments for critically ill patients. This approach will enhance patient outcomes and reduce medical resource waste, offering reliable sensitivity and specificity to support precision medicine in critical care.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70235","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T06:31:42Z","timestamp":1761028302000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Establishing Plasma Concentration Methodology for Trimethoprim\/Sulfamethoxazole: Application in Therapeutic Drug Monitoring of Critically Ill Patients"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1446-0901","authenticated-orcid":false,"given":"Xiangxiang","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Pharmacy Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University)  Haikou Hainan China"},{"name":"School of Pharmacy Hainan Medical University  Haikou Hainan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiao","family":"Fu","sequence":"additional","affiliation":[{"name":"Central Laboratory Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University)  Haikou Hainan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haibiao","family":"Gong","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Research Center of Traditional Chinese Medicine &amp; Disease Susceptibility\/State Key Laboratory of Bioactive Molecules and Druggability Assessment, College of Pharmacy Jinan University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University)  Haikou Hainan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"FangLing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University)  Haikou Hainan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MengYu","family":"Mei","sequence":"additional","affiliation":[{"name":"School of Pharmacy Hainan Medical University  Haikou Hainan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University)  Haikou Hainan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenya","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University)  Haikou Hainan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University)  Haikou Hainan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanyang","family":"Sun","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Research Center of Traditional Chinese Medicine &amp; 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