{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T05:52:05Z","timestamp":1779083525449,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T00:00:00Z","timestamp":1761264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Several studies have reported detection methods for etomidate and its analogues, metomidate and propoxate, but data on their pharmacokinetics and bioavailability are limited. This study develops a UPLC\u2013MS\/MS method to simultaneously detect these compounds and evaluates their pharmacokinetics and bioavailability in mice. Plasma samples were processed using methanol\u2010induced protein precipitation. The separation was conducted on a UPLC BEH C18 column with an acetonitrile\u2010water (containing 0.1% formic acid) as mobile phase at a flow rate of 0.4\u2009mL\/min, achieving elution within 4\u2009min. Quantitative analysis was performed using MRM mode coupled with ESI in positive ion mode. In this study, mice received etomidate, metomidate, and propoxate via intravenous (1\u2009mg\/kg) and oral (10\u2009mg\/kg) routes, and the pharmacokinetics were evaluated. The calibration curves demonstrated excellent linearity within the ranges of 0.5\u20131000\u2009ng\/mL for etomidate, 0.504\u20131080\u2009ng\/mL for metomidate, and 0.77\u20131540\u2009ng\/mL for propoxate in mouse plasma, with correlation coefficients (\n                    <jats:italic>r<\/jats:italic>\n                    values) exceeding 0.998. The developed UPLC\u2010MS\/MS method was successfully utilized to analyze the pharmacokinetics of etomidate, metomidate, and propoxate. The absolute bioavailability of etomidate, metomidate, and propoxate was determined to be 14.0%, 21.3%, and 15.3%, respectively.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70242","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T13:26:22Z","timestamp":1761312382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pharmacokinetic and Bioavailability Analysis of Etomidate, Metomidate, and Propoxate in Mice Using UPLC\u2013MS\/MS"],"prefix":"10.1002","volume":"39","author":[{"given":"Jianshe","family":"Ma","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siyu","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siyuan","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xicheng","family":"Dong","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3494-2405","authenticated-orcid":false,"given":"Lufeng","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9821-3770","authenticated-orcid":false,"given":"Xianqin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Wenzhou Medical University  Wenzhou China"},{"name":"Department of Neurosurgery, Wenzhou Municipal Key Laboratory of Neurodevelopmental Pathology and Physiology The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15563650.2024.2423834"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph18020250"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11419\u2010023\u201000678\u20106"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201057581\u20102"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102885.2003.00454.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2025.114659"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcemcr\/luae207"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/d4an01213e"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2024.124340"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2025.116962"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/1567201818666210617101630"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2024.107503"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3869"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262\u2010021\u201001038\u20106"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.116094"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2024.1321610"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1556\u20104029.14823"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11419\u2010024\u201000707\u2010y"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1556\/1326.2024.01158"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.4c00325"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2024.124374"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70242","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T12:53:08Z","timestamp":1764420788000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70242"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,24]]},"references-count":21,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/bmc.70242"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70242","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,24]]},"assertion":[{"value":"2025-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70242"}}