{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:34:50Z","timestamp":1786977290857,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A simple, sensitive and efficient ultra performance liquid chromatography\u2013mass spectrometry (UPLC\u2013MS\/MS) method for simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma has been developed and validated. Using olmesartan\u2010d\n                    <jats:sub>4<\/jats:sub>\n                    and hydrochlorothiazide\u2010\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010d\n                    <jats:sub>2<\/jats:sub>\n                    as stable isotope\u2010labeled internal standard (SIL\u2010IS). Plasma samples were processed by protein precipitation. Separated on a ZORBAX Eclipse XDB\u2010Phenyl column (150\u2009mm\u2009\u00d7\u20094.6\u2009mm, 5\u2009\u03bcm) using a gradient elution with mobile phases methanol and 5\u2009mM ammonium acetate. Detected by electrospray ionization (ESI) in negative ion mode of multiple reaction monitoring (MRM), mass transition ion pairs were\n                    <jats:italic>m\/z<\/jats:italic>\n                    445.1\u2009\u2192\u2009149.0 for olmesartan,\n                    <jats:italic>m\/z<\/jats:italic>\n                    295.9\u2009\u2192\u2009204.9 for hydrochlorothiazide,\n                    <jats:italic>m\/z<\/jats:italic>\n                    449.1\u2009\u2192\u2009149.1 for olmesartan\u2010d\n                    <jats:sub>4<\/jats:sub>\n                    , and\n                    <jats:italic>m\/z<\/jats:italic>\n                    301.0\u2009\u2192\u2009272.0 for hydrochlorothiazide\u2010\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010d\n                    <jats:sub>2<\/jats:sub>\n                    . Linear ranges were 10\u20132000\u2009ng\/mL for olmesartan and 1.5\u2013300\u2009ng\/mL for hydrochlorothiazide. Mean recoveries of olmesartan, hydrochlorothiazide, olmesartan\u2010d\n                    <jats:sub>4<\/jats:sub>\n                    , and hydrochlorothiazide\u2010\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010d\n                    <jats:sub>2<\/jats:sub>\n                    were 91.31%, 99.34%, 92.62%, and 98.61%, respectively. Our method was well validated in selectivity, carryover, lower limit of quantification (LLOQ), calibration curve, accuracy, precision, dilution effect, matrix effect (normal, hyperlipidemic, and hemolyzed matrices), stability, recovery, and IRS. It was successfully applied in a bioequivalence study of olmesartan medoxomil and hydrochlorothiazide tablets (20\/12.5\u2009mg) in healthy Chinese volunteers.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70254","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T07:52:31Z","timestamp":1764402751000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Simultaneous Determination of Olmesartan and Hydrochlorothiazide in Human Plasma by UPLC\u2013MS\/MS Method and Its Application to a Bioequivalence Study"],"prefix":"10.1002","volume":"40","author":[{"given":"Jie","family":"Shen","sequence":"first","affiliation":[{"name":"Anhui Provincial Center of Drug Clinical Evaluation Yijishan Hospital of Wannan Medical College  Wuhu Anhui People's Republic of China"},{"name":"School of Pharmacy Wannan Medical College  Wuhu Anhui People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6855-165X","authenticated-orcid":false,"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Wannan Medical College  Wuhu Anhui People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbang","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Wannan Medical College  Wuhu Anhui People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Anhui Wanbang Pharmaceutical Technology Co. 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